Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hsp60 antibody [LK-2] (ab59458)

Key features and details

  • Mouse monoclonal [LK-2] to Hsp60
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Hsp60 antibody [LK-2]
    See all Hsp60 primary antibodies

  • Description

    Mouse monoclonal [LK-2] to Hsp60

  • Host species

    Mouse

  • Specificity

    Detects ~60kDa.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Human Hsp60 produced through recombinant DNA methods in E.coli

  • Positive control

    • HeLa cell lysate

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

    Immunohistochemistry analysis of  Paraffin Embedded Human kidney tissue using Mouse Anti-HSP60 Antibody, Clone LK2 (SMC-111) at 1/100 dilution.

     

  • Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
    Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
    Anti-Hsp60 antibody [LK-2] (ab59458) at 1/1000 dilution + HeLa cell lysate

    Predicted band size: 60 kDa

  • Western blot - Anti-Hsp60 antibody [LK-2] (ab59458)
    Western blot - Anti-Hsp60 antibody [LK-2] (ab59458) This image is courtesy of an anonymous Abreview
    Lanes 1-3 : Anti-Hsp60 antibody [LK-2] (ab59458) at 1/4000 dilution


    Lanes 1-3 : Saccharomyces cerevisiae whole cell lysate at 6900000 cells
    Lane 4 : Ladder

    Secondary
    Lanes 1-2 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/5000 dilution
    Lane 3 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/2000 dilution


    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa


    Exposure time: 25 seconds

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

    ab59464 at 100,000 dilution staining Hsp60 alpha in human colon tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp60 antibody [LK-2] (ab59458)

    Paraffin-embedded mouse back skin tissue stained for Hsp60 using ab59458 at 1/100 dilution in immunohistochemical analysis. DAB staining.

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