Anti-PCNA antibody [16D10] (ab252848)
![Anti-PCNA antibody [16D10] (ab252848)](https://www.abcam.com/ps/products/252/ab252848/Images/ab252848-397071-anti-pcna-antibody-16d10-immunocytochemistry-nih3t3-cells.jpg "Anti-PCNA antibody [16D10] (ab252848)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [16D10] to PCNA
- Suitable for: ICC, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PCNA antibody [16D10]
See all PCNA primary antibodies -
Description
Rat monoclonal [16D10] to PCNA -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species ICC MouseRatHumanIP Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: NIH/3T3, HeLa and C6 cells. IP: NIH/3T3 cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
16D10 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry - Anti-PCNA antibody [16D10] (ab252848)
Immunocytochemical analysis of 100% methanol-fixed NIH/3T3 (mouse embryonic fibroblast) cells labeling PCNA (green) using ab252848 at 7.08µg/ml followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in NIH/3T3 cell line. The nuclear counterdstain was DAPI (Blue).
-ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).
-ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).
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![Immunoprecipitation - Anti-PCNA antibody [16D10] (ab252848)](https://www.abcam.com/ps/products/252/ab252848/Images/ab252848-397074-anti-pcna-antibody-16d10-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PCNA antibody [16D10] (ab252848)PCNA was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate using ab252848 at 1/30 dilution (2µg in 0.35µg lysates). Western blot was performed on the immunoprecipitate using ab252848 at 1/1000 dilution (0.35µg/ml) followed by anti-Rat IgG H&L (HRP) (ab205720) secondary at 1/5000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 µg.
Lane 2: ab252848 IP in NIH/3T3 whole cell lysate.
Lane 3: Rat monoclonal IgG instead of ab252848 in NIH/3T3 whole cell lysate.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Immunocytochemistry - Anti-PCNA antibody [16D10] (ab252848)](https://www.abcam.com/ps/products/252/ab252848/Images/ab252848-397072-anti-pcna-antibody-16d10-immunocytochemistry-c6-cells.jpg)
Immunocytochemistry - Anti-PCNA antibody [16D10] (ab252848)Immunocytochemical analysis of 100% methanol-fixed C6 (rat glial tumor glial cell) cells labeling PCNA (green) using ab252848 at 7.08µg/ml followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in C6 cell line. The nuclear counterdstain was DAPI (Blue).
-ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).
-ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).
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![Immunocytochemistry - Anti-PCNA antibody [16D10] (ab252848)](https://www.abcam.com/ps/products/252/ab252848/Images/ab252848-397073-anti-pcna-antibody-16d10-immunocytochemistry-hela-cells.jpg)
Immunocytochemistry - Anti-PCNA antibody [16D10] (ab252848)Immunocytochemical analysis of 100% methanol-fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PCNA (green) using ab252848 at 7.08µg/ml followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2µg/ml). ab179504 Anti-beta IV Tubulin antibody was used as a counterstain at 1/200 dilution (10µg/ml) followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (2µg/ml) (Red). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterdstain was DAPI (Blue).
-ve control 1: ab252848 (1/50 dilution) followed by ab150080 (1/1000 dilution).
-ve control 2: ab179504 (1/200 dilution) followed by ab150157 (1/1000 dilution).
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![Anti-PCNA antibody [16D10] (ab252848)](https://www.abcam.com/ps/products/252/ab252848/Images/ab252848-5-benefits-of-recombinant-antibodies.png)
Anti-PCNA antibody [16D10] (ab252848)
