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Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12326] to FUBP1/FBP
  • Suitable for: WB, IHC-P, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-FUBP1/FBP antibody [EPR12326]
    See all FUBP1/FBP primary antibodies

  • Description

    Rabbit monoclonal [EPR12326] to FUBP1/FBP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat and HepG2 whole cell lysate (ab7900). IHC-P: Human endometrium and ovarian adenocarcinoma tissues. ICC/IF: HeLa cells. IP: FUBP1/FBP IP in HeLa cell lysate.

  • General notes

    Previously labeled as FUBP1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
    Western blot - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
    All lanes : Anti-FUBP1/FBP antibody [EPR12326] (ab192867) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

    Immunohistochemical analysis of paraffin embedded human endometrium tissue labeling FUBP1/FBP using ab192867 at a 1/2000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

    Immunohistochemical analysis of paraffin embedded human ovarian adenocarcinoma tissue labeling FUBP1/FBP using ab192867 at a 1/2000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
    Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP using ab192867 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary antibody, at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

  • Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
    Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

    Western blot analysis of pellet from HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate immunoprecipitated using ab192867 at a 1/100 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.

  • Anti-FUBP1/FBP antibody [EPR12326] (ab192867)
    Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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