Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12326] to FUBP1/FBP
Suitable for: WB, IHC-P, IP, ICC/IF
Reacts with: Human

Product name

Anti-FUBP1/FBP antibody [EPR12326]
See all FUBP1/FBP primary antibodies
Description

Rabbit monoclonal [EPR12326] to FUBP1/FBP
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, Jurkat and HepG2 whole cell lysate (ab7900). IHC-P: Human endometrium and ovarian adenocarcinoma tissues. ICC/IF: HeLa cells. IP: FUBP1/FBP IP in HeLa cell lysate.
General notes
Previously labeled as FUBP1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12326
Isotype

IgG
Research areas

Western blot - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

All lanes : Anti-FUBP1/FBP antibody [EPR12326] (ab192867) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa
why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

Immunohistochemical analysis of paraffin embedded human endometrium tissue labeling FUBP1/FBP using ab192867 at a 1/2000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

Immunohistochemical analysis of paraffin embedded human ovarian adenocarcinoma tissue labeling FUBP1/FBP using ab192867 at a 1/2000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP using ab192867 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor^®488) (ab150077) was used as the secondary antibody, at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.
Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

Western blot analysis of pellet from HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate immunoprecipitated using ab192867 at a 1/100 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.
Anti-FUBP1/FBP antibody [EPR12326] (ab192867)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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