Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

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Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19208] to FUBP1/FBP
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-FUBP1/FBP antibody [EPR19208]
See all FUBP1/FBP primary antibodies
Description

Rabbit monoclonal [EPR19208] to FUBP1/FBP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Mouse Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat, HeLa, Raji, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates; rat and mouse brain lysates. IHC-P: Human oligodendroglioma and cerebral cortex tissues; mouse cerebral cortex tissue; rat stomach tissue. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
General notes
Previously labelled as FUBP1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19208
Isotype

IgG
Research areas

Western blot - Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

All lanes : Anti-FUBP1/FBP antibody [EPR19208] (ab213525) at 1/5000 dilution

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression pattern observed is consistent with the literature (PMID: 24963357).
Western blot - Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

Lanes 1-2 : Anti-FUBP1/FBP antibody [EPR19208] (ab213525) at 1/1000 dilution
Lanes 3-6 : Anti-FUBP1/FBP antibody [EPR19208] (ab213525) at 1/5000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 68 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-3/5/6: 1 second; Lane 4: 3 seconds.

The expression pattern observed is consistent with the literature (PMID: 24963357).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

Immunohistochemical analysis of paraffin-embedded human oligodendroglioma tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the vascular endothelium of human oligodendroglioma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the neurons of human cerebral cortex is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the neurons of mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling FUBP1/FBP with ab213525 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the neurons of rat stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP with ab213525 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling FUBP1/FBP with ab213525 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Flow Cytometry - Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling FUBP1/FBP with ab213525 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

FUBP1/FBP was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab213525 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213525 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: HeLa whole cell lysate 10µg (Input).

Lane 2: ab213525 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213525 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.

The expression pattern observed is consistent with the literature (PMID: 24963357).
Anti-FUBP1/FBP antibody [EPR19208] (ab213525)

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