Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)]
See all Zinc Alpha 2 Glycoprotein primary antibodies
Description

Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human Plasma and HepG2 cell lines; Human liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14425(B)
Isotype

IgG
Research areas

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/20000 dilution + Human plasma at 20 µg

Secondary
Anti-rabbit (HRP) specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/2000 dilution + HepG2 at 10 µg

Secondary
Goat Anti-rabbit HRP at 1/1000 dilution

Predicted band size: 34 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

Immunoprecipitation of Human plasma labeling Zinc Alpha 2 Glycoprotein using ab180574at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500.

Blocking/dilution buffer and concentration: 5% NFDM/TBST

All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/30 dilution

Lane 1 : Human plasma
Lane 2 : Negative control

Secondary
All lanes : Anti-Rabbit (HRP) specific to the non-reduced form of IgG at 1/500 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

Immunohistochemical analysis of Human liver tissue labeling Zinc Alpha 2 Glycoprotein with ab180574 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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