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Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)]
    See all Zinc Alpha 2 Glycoprotein primary antibodies

  • Description

    Rabbit monoclonal [EPR14425(B)] to Zinc Alpha 2 Glycoprotein

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human Plasma and HepG2 cell lines; Human liver tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14425(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)
    Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)
    Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/20000 dilution + Human plasma at 20 µg

    Secondary
    Anti-rabbit (HRP) specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 34 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)
    Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)
    Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/2000 dilution + HepG2 at 10 µg

    Secondary
    Goat Anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 34 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)
    Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

    Immunoprecipitation of Human plasma labeling Zinc Alpha 2 Glycoprotein using ab180574at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500.

    Blocking/dilution buffer and concentration: 5% NFDM/TBST



    All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574) at 1/30 dilution

    Lane 1 : Human plasma
    Lane 2 : Negative control

    Secondary
    All lanes : Anti-Rabbit (HRP) specific to the non-reduced form of IgG at 1/500 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

    Immunohistochemical analysis of Human liver tissue labeling Zinc Alpha 2 Glycoprotein with ab180574 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)
    Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (ab180574)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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