Anti-CTGF antibody (ab5097)
Key features and details
- Rabbit polyclonal to CTGF
- Suitable for: ICC, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-CTGF antibody
See all CTGF primary antibodies -
Description
Rabbit polyclonal to CTGF -
Host species
Rabbit -
Specificity
This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741). We have been unable to detect endogenous protein in WB with ab5097. -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Recombinant fragment
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Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC HumanWB Recombinant fragmentAll applications RatPigApplication Abreviews Notes ICC Use a concentration of 1 µg/ml.WB 1/500. Predicted molecular weight: 38 kDa.This application has only been tested on recombinant protein.
Notes ICC
Use a concentration of 1 µg/ml.WB
1/500. Predicted molecular weight: 38 kDa.This application has only been tested on recombinant protein.
Target
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Function
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. -
Tissue specificity
Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1490 Human
- Entrez Gene: 100152141 Pig
- Entrez Gene: 64032 Rat
- Omim: 121009 Human
- SwissProt: P29279 Human
- SwissProt: O19113 Pig
- SwissProt: Q9R1E9 Rat
- Unigene: 410037 Human
see all -
Alternative names
- CCN 2 antibody
- CCN family member 2 antibody
- CCN2 antibody
see all
Images
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Immunocytochemistry - Anti-CTGF antibody (ab5097)
ab5097 staining CTGF in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5097 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-CTGF antibody (ab5097)Western blot, on recombinant Human CTGF produced in E. coli, using ab5097 at 1/500.
Not yet tested against endogenous CTGFSecondary antibody - anti-rabbit HRP (ab6721)
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Immunocytochemistry - Anti-CTGF antibody (ab5097)This image is courtesy of an Abreview submitted by Beate Thodeab5097 staining CTGF in Human Hela, cervix cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. A Cy3®-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/800).
Protocols
References (14)
ab5097 has been referenced in 14 publications.
- Li L et al. MicroRNA-584 Impairs Cellular Proliferation and Sensitizes Osteosarcoma Cells to Cisplatin and Taxanes by Targeting CCN2. Cancer Manag Res 12:2577-2587 (2020). PubMed: 32346311
- Sourbier C et al. Targeting loss of the Hippo signaling pathway in NF2-deficient papillary kidney cancers. Oncotarget 9:10723-10733 (2018). PubMed: 29535838
- Lambers E et al. Foxc1 Regulates Early Cardiomyogenesis and Functional Properties of Embryonic Stem Cell Derived Cardiomyocytes. Stem Cells 34:1487-500 (2016). PubMed: 26824887
- Lee CH et al. Harnessing endogenous stem/progenitor cells for tendon regeneration. J Clin Invest 125:2690-701 (2015). PubMed: 26053662
- Meng YH et al. Elevated expression of connective tissue growth factor, osteopontin and increased collagen content in human ascending thoracic aortic aneurysms. Vascular 22:20-7 (2014). IHC ; Human . PubMed: 23508392
Images
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Immunocytochemistry - Anti-CTGF antibody (ab5097)
ab5097 staining CTGF in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5097 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-CTGF antibody (ab5097)
Western blot, on recombinant Human CTGF produced in E. coli, using ab5097 at 1/500.
Not yet tested against endogenous CTGFSecondary antibody - anti-rabbit HRP (ab6721)
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Immunocytochemistry - Anti-CTGF antibody (ab5097) This image is courtesy of an Abreview submitted by Beate Thode
ab5097 staining CTGF in Human Hela, cervix cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. A Cy3®-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/800).
