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Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10954] to INPPL1/SHIP-2
  • Suitable for: WB, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-INPPL1/SHIP-2 antibody [EPR10954]
    See all INPPL1/SHIP-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10954] to INPPL1/SHIP-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human INPPL1/SHIP-2.
    Database link: O15357

  • Positive control

    • Daudi, HeLa and K562 cell lysates; Permeabilized K562 cells.

  • General notes

    Previously labelled as INPPL1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)
    Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: INPPL1/SHIP-2 knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: K562 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab157460 observed at 139 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab157460 was shown to specifically react with INPPL1/SHIP-2 in wild-type HAP1 cells. No bands were observed when INPPL1/SHIP-2 knockout samples were examined. Wild-type and INPPL1/SHIP-2 knockout samples were subjected to SDS-PAGE.  Ab157460 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)
    Flow Cytometry - Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)

    Flow cytometric analysis of permeabilized K562 cells labeling INPPL1/SHIP-2 with ab157460 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)
    Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)
    All lanes : Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 139 kDa

  • Immunoprecipitation - Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)
    Immunoprecipitation - Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)

    Detection of INPPL1/SHIP-2 by Western Blot of Immunprecipitate. HeLa cell lysate immunoprecipitated using ab157460 at 1/10 dilution.



    Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate
  • Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)
    Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460)

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