Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)
![Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)](https://www.abcam.com/ps/products/166/ab166916/Images/ab166916-282522-anti-inppl1-ship-2-antibody-epr10955-western-blot.jpg "Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10955] to INPPL1/SHIP-2
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-INPPL1/SHIP-2 antibody [EPR10955]
See all INPPL1/SHIP-2 primary antibodies -
Description
Rabbit monoclonal [EPR10955] to INPPL1/SHIP-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human INPPL1/SHIP-2 aa 950-1050. The exact sequence is proprietary.
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Positive control
- K562, HeLa whole cell lysate (ab150035); Human heart tissue; Human kidney tissue; HeLa cells
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General notes
Previously labelled as INPPL1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10955 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: INPPL1/SHIP-2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab166916 observed at 139 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab166916 was shown to specifically react with INPPL1/SHIP-2 in wild-type HAP1 cells. No band was observed when INPPL1/SHIP-2 knockout samples were examined. Wild-type and INPPL1/SHIP-2 knockout samples were subjected to SDS-PAGE. Ab166916 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)](https://www.abcam.com/ps/products/166/ab166916/Images/ab166916-165292-anti-inppl1-ship-2-antibody-epr10955-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling INPPL1/SHIP-2 with ab166916 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)](https://www.abcam.com/ps/products/166/ab166916/Images/ab166916-165293-anti-inppl1-ship-2-antibody-epr10955-western-blot.jpg)
Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)All lanes : Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 139 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)](https://www.abcam.com/ps/products/166/ab166916/Images/ab166916-165291-anti-inppl1-ship-2-antibody-epr10955-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling INPPL1/SHIP-2 with ab166916 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)](https://www.abcam.com/ps/products/166/ab166916/Images/ab166916-165290-anti-inppl1-ship-2-antibody-epr10955-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)Immunofluorescent analysis of HeLa cells labeling INPPL1/SHIP-2 with ab166916 at 1/100 dilution.
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![Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)](https://www.abcam.com/ps/products/166/ab166916/Images/ab166916-6-benefits-of-recombinant-antibodies.png)
Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)
