Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10955] to INPPL1/SHIP-2
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-INPPL1/SHIP-2 antibody [EPR10955]
    See all INPPL1/SHIP-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10955] to INPPL1/SHIP-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human INPPL1/SHIP-2 aa 950-1050. The exact sequence is proprietary.

  • Positive control

    • K562, HeLa whole cell lysate (ab150035); Human heart tissue; Human kidney tissue; HeLa cells

  • General notes

    Previously labelled as INPPL1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)
    Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: INPPL1/SHIP-2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: K562 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab166916 observed at 139 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab166916 was shown to specifically react with INPPL1/SHIP-2 in wild-type HAP1 cells. No band was observed when INPPL1/SHIP-2 knockout samples were examined. Wild-type and INPPL1/SHIP-2 knockout samples were subjected to SDS-PAGE. Ab166916 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling INPPL1/SHIP-2 with ab166916 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)
    Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)
    All lanes : Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 139 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling INPPL1/SHIP-2 with ab166916 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)
    Immunocytochemistry/ Immunofluorescence - Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)

    Immunofluorescent analysis of HeLa cells labeling INPPL1/SHIP-2 with ab166916 at 1/100 dilution.

  • Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)
    Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-INPPL1/SHIP-2 antibody [EPR10955] (ab166916)

© 2021 Astana Biomed Group LLP. All rights reserved.