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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-INPPL1/SHIP-2 antibody (ab70267)

Anti-INPPL1/SHIP-2 antibody (ab70267)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to INPPL1/SHIP-2
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-INPPL1/SHIP-2 antibody
    See all INPPL1/SHIP-2 primary antibodies
  • Description

    Rabbit polyclonal to INPPL1/SHIP-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human INPPL1/SHIP-2 aa 100-150.
    Database link: NP_001558.2

  • General notes

    Previously labelled as INPPL1. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Lipid Phosphatases
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Neuroscience
    • Neurology process
    • Metabolism
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody (ab70267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody (ab70267)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling INPPL1/SHIP-2 with ab70267 at 1/1000 (1µg/ml). Detection: DAB.

  • Western blot - Anti-INPPL1/SHIP-2 antibody (ab70267)
    Western blot - Anti-INPPL1/SHIP-2 antibody (ab70267)
    Anti-INPPL1/SHIP-2 antibody (ab70267) at 0.1 µg/ml + HeLa whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 139 kDa
    Observed band size: 139 kDa


    Exposure time: 10 seconds
  • Immunoprecipitation - Anti-INPPL1/SHIP-2 antibody (ab70267)
    Immunoprecipitation - Anti-INPPL1/SHIP-2 antibody (ab70267)
    Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70267 at 3 µg/mg. 1/4 of the immunoprecipitate was loaded on to a gel and analysed by Western blot using ab70267 at 0.1 µg/ml. Blot was developed using chemiluminescence with an exposure time of 30 seconds.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody (ab70267)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPPL1/SHIP-2 antibody (ab70267)
    IHC image of ab70267 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70267, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: ICC/IF, IHC-P, WB

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