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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-CTGF antibody [17-73-F1] (ab94939)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-CTGF antibody [17-73-F1] (ab94939)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [17-73-F1] to CTGF
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG2

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Overview

  • Product name

    Anti-CTGF antibody [17-73-F1]
    See all CTGF primary antibodies
  • Description

    Mouse monoclonal [17-73-F1] to CTGF
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Pig, Baboon
  • Immunogen

    Synthetic peptide corresponding to Human CTGF aa 150-250. corresponding to the hinge region of Human CTGF
    Database link: P29279

  • Positive control

    • This antibody gave a positive signal when tested against recombinant CTGF protein, and in the following lysates: HeLa whole cell; HEK293 whole cell; Bone marrow (human) tissue; ovary (human) tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    17-73-F1
  • Isotype

    IgG2
  • Light chain type

    lambda
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Immunology
    • Secreted Molecules
    • CCN Family

Images

  • Western blot - Anti-CTGF antibody [17-73-F1] (ab94939)
    Western blot - Anti-CTGF antibody [17-73-F1] (ab94939)
    Anti-CTGF antibody [17-73-F1] (ab94939) at 10 µg/ml + CTGF Recombinant Protein (Human) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa


    Exposure time: 4 minutes


    The human CTGF His-tagged fusion protein, produced in E. coli, is 38.3KDa protein containing 323 amino acid residues of the human CTGF and 23 additional amino acid residues- His Tag, Xa- cleavage site.
  • Western blot - Anti-CTGF antibody [17-73-F1] (ab94939)
    Western blot - Anti-CTGF antibody [17-73-F1] (ab94939)
    All lanes : Anti-CTGF antibody [17-73-F1] (ab94939) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 4 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa
    Additional bands at: 44 kDa (possible glycosylated form), 50 kDa, 60 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    CTGF contains two glycosylation sites (SwissProt) which may explain the higher migrating band.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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