Anti-CTGF antibody [17-73-F1] (ab94939)
Key features and details
- Mouse monoclonal [17-73-F1] to CTGF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2
Overview
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Product name
Anti-CTGF antibody [17-73-F1]
See all CTGF primary antibodies -
Description
Mouse monoclonal [17-73-F1] to CTGF -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Pig, Baboon -
Immunogen
Synthetic peptide corresponding to Human CTGF aa 150-250. corresponding to the hinge region of Human CTGF
Database link: P29279 -
Positive control
- This antibody gave a positive signal when tested against recombinant CTGF protein, and in the following lysates: HeLa whole cell; HEK293 whole cell; Bone marrow (human) tissue; ovary (human) tissue.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
17-73-F1 -
Isotype
IgG2 -
Light chain type
lambda -
Research areas
Images
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Anti-CTGF antibody [17-73-F1] (ab94939) at 10 µg/ml + CTGF Recombinant Protein (Human) at 0.1 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 4 minutes
The human CTGF His-tagged fusion protein, produced in E. coli, is 38.3KDa protein containing 323 amino acid residues of the human CTGF and 23 additional amino acid residues- His Tag, Xa- cleavage site. -
All lanes : Anti-CTGF antibody [17-73-F1] (ab94939) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 4 : Human ovary tissue lysate - total protein (ab30222)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 44 kDa (possible glycosylated form), 50 kDa, 60 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
CTGF contains two glycosylation sites (SwissProt) which may explain the higher migrating band.