Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CTGF antibody [EPR20728] - BSA and Azide free (ab231824)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20728] to CTGF - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CTGF antibody [EPR20728] - BSA and Azide free
    See all CTGF primary antibodies

  • Description

    Rabbit monoclonal [EPR20728] to CTGF - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Starved NIH/3T3 cells treated with TGF-ß1 and Heparin sodium salt whole cell lysate. HepG2 whole cell lysate.

  • General notes

    Ab231824 is the carrier-free version of ab209780. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231824 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CTGF antibody [EPR20728] - BSA and Azide free (ab231824)
    Immunoprecipitation - Anti-CTGF antibody [EPR20728] - BSA and Azide free (ab231824)

    CTGF was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab209780 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209780 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 µg (Input).
    Lane 2: ab209780 IP in HepG2 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209780 in HepG2 whole cell lysate (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The molecular mass observed is consistent with the literature. (PMID:27126736)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209780).

  • Western blot - Anti-CTGF antibody [EPR20728] - BSA and Azide free (ab231824)
    Western blot - Anti-CTGF antibody [EPR20728] - BSA and Azide free (ab231824)
    All lanes : Anti-CTGF antibody [EPR20728] (ab209780) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) starved for 18 hours, whole cell lysate
    Lane 2 : NIH/3T3 starved for 18 hours, then treated with 10 ng/ml transforming growth factor-ß (TGF-ß1, ab50036) and 50 µg/ml Heparin sodium salt for 24 hours, whole cell lysate
    Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa



    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    The level of CTGF expression can be induced by TGFß treatment (PMID: 17786299).

    CTGF is constitutively expressed in HepG2 cells (PMID:15886528).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209780).

     

  • Anti-CTGF antibody [EPR20728] - BSA and Azide free (ab231824)
    Anti-CTGF antibody [EPR20728] - BSA and Azide free (ab231824)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CTGF antibody [EPR20728] - BSA and Azide free (ab231824)

© 2021 Astana Biomed Group LLP. All rights reserved.