Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell lysate (ab257084)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263472 - Human CA2 knockout HEK293T cell lysate 1 x 100µg ab255594 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 11, 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 15, 20 TH01: 7, 9.3 TPOX: 11, 12 CSF1PO: 12
Images
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Western blot - Human CA2 knockout HEK293T cell lysate (ab257084)
Lane 1:Wild-type HEK293T cell lysate (20 ug)
Lane 2:CA2 knockout HEK293T cell lysate (20 ug)
Lane 3:A431 cell lysate (20 ug)
Lane 4:Caco-2 cell lysate (20 ug)ab82559 was shown to specifically react with Carbonic anhydrase 2/CA2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265072 (knockout cell lysate ab257084) was used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab82559 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human CA2 knockout HEK293T cell lysate (ab257084)Lane 1:Wild-type HEK293T cell lysate (20 ug)
Lane 2:CA2 knockout HEK293T cell lysate (20 ug)
Lane 3:A431 cell lysate (20 ug)
Lane 4:Caco-2 cell lysate (20 ug)ab124687 was shown to specifically react with Carbonic anhydrase 2/CA2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265072 (knockout cell lysate ab257084) was used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab124687 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human CA2 knockout HEK293T cell lysate (ab257084)Homozygous: 1 bp insertion in exon 1
