Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)
![Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)](https://www.abcam.com/ps/products/250/ab250980/Images/ab250980-4-anti-lime-antibody-epr14713-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif "Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14713] to LIME - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-LIME antibody [EPR14713] - BSA and Azide free
See all LIME primary antibodies -
Description
Rabbit monoclonal [EPR14713] to LIME - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250980 is the carrier-free version of ab188871. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250980 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR14713 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)This data was developed using ab188871, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human mammary gland infiltrating duct carcinoma tissue labeling LIME with ab188871 at 1/2000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)](https://www.abcam.com/ps/products/250/ab250980/Images/ab250980-3-anti-lime-antibody-epr14713-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)This data was developed using ab188871, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling LIME with ab188871 at 1/2000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Western blot - Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)](https://www.abcam.com/ps/products/250/ab250980/Images/ab250980-402868-anti-lime-antibody-epr14713-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)Anti-LIME antibody [EPR14713] (ab188871) at 1/1000 dilution + Human thymus lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution
Predicted band size: 31 kDaThis data was developed using ab188871, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)](https://www.abcam.com/ps/products/250/ab250980/Images/ab250980-398992-anti-lime-antibody-epr14713-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980) -
![Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)](https://www.abcam.com/ps/products/250/ab250980/Images/ab250980-5-benefits-of-recombinant-antibodies.png)
Anti-LIME antibody [EPR14713] - BSA and Azide free (ab250980)