Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD1c antibody [EPR23189-196] (ab246520)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23189-196] to CD1c
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD1c antibody [EPR23189-196]
    See all CD1c primary antibodies

  • Description

    Rabbit monoclonal [EPR23189-196] to CD1c

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat and MOLT-4 whole cell lysates; Human thymus, tonsil and spleen tissue lysates. IHC-P: Human spleen and Hodgkin's lymphoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23189-196

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (ab246520)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (ab246520)

    Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CD1c with ab246520 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human Hodgkin's lymphoma. The section was incubated with ab246520 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520)
    Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520)
    All lanes : Anti-CD1c antibody [EPR23189-196] (ab246520) at 1/1000 dilution

    Lane 1 : Human thymus tissue lysate
    Lane 2 : Human tonsil tissue lysate
    Lane 3 : Human spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 1385541).

    Exposure times: Lane 1: 15 seconds; Lane 2: 37 seconds; Lanes 3: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (ab246520)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (ab246520)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD1c with ab246520 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen. (PMID: 15191950)The section was incubated with ab246520 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520)
    Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520)
    All lanes : Anti-CD1c antibody [EPR23189-196] (ab246520) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 38 kDa
    Observed band size: 43 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 1385541).

    Negative control: THP-1 (PMID: 24935257)

    There is an unidentifiable protein at high molecular weight. 

    Exposure time: 26 seconds.

  • Anti-CD1c antibody [EPR23189-196] (ab246520)
    Anti-CD1c antibody [EPR23189-196] (ab246520)

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