Anti-PKN2 antibody [EPR5490] - BSA and Azide free (ab248857)
![Anti-PKN2 antibody [EPR5490] - BSA and Azide free (ab248857)](https://www.abcam.com/ps/products/248/ab248857/Images/ab248857-412725-anti-pkn2-antibody-epr5490-western-blot-human-knockout-lysate.jpg "Anti-PKN2 antibody [EPR5490] - BSA and Azide free (ab248857)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5490] to PKN2 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PKN2 antibody [EPR5490] - BSA and Azide free
See all PKN2 primary antibodies -
Description
Rabbit monoclonal [EPR5490] to PKN2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HCT116, HEK-293T, Jurkat, HeLa, and HepG2 (ab7900) cell lysates. ICC/IF: HeLa cells.
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General notes
ab248857 is the carrier-free version of ab138514.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR5490 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PKN2 antibody [EPR5490] - BSA and Azide free (ab248857)All lanes : Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution
Lane 1 : Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 2 : PKN2 knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 112 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?This data was developed using ab138514, the same antibody clone in a different buffer formulation.
Lanes 1-3: Merged signal (red and green). Green - ab138514 observed at 125 kDa. Red - loading control ab8245 observed at 36 kDa.
ab138514 Anti-PKN2 antibody [EPR5490] (ab138514) was shown to specifically react with PKN2 in wild-type HCT cells. Loss of signal was observed when knockout cell line ab266894 (knockout cell lysate ab258588) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PKN2 antibody [EPR5490] - BSA and Azide free (ab248857)](https://www.abcam.com/ps/products/248/ab248857/Images/ab248857-383551-anti-pkn2-antibody-epr5490-bsa-and-azide-free-western-blot-hela-293t.jpg)
Western blot - Anti-PKN2 antibody [EPR5490] - BSA and Azide free (ab248857)All lanes : Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PKN2 knockout HeLa cell lysate
Lane 3 : HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 112 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab138514).
Lanes 1-3: Merged signal (red and green). Green - ab138514 observed at 112 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab138514 Anti-PKN2 antibody [EPR5490] was shown to specifically react with PKN2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264691 (knockout cell lysate ab258587) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Anti-PKN2 antibody [EPR5490] - BSA and Azide free (ab248857)](https://www.abcam.com/ps/products/248/ab248857/Images/ab248857-3-benefits-of-recombinant-antibodies.png)
Anti-PKN2 antibody [EPR5490] - BSA and Azide free (ab248857)
