Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1488Y] to PKC theta/PRKCQ
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PKC theta/PRKCQ antibody [EPR1488Y]
See all PKC theta/PRKCQ primary antibodies -
Description
Rabbit monoclonal [EPR1488Y] to PKC theta/PRKCQ -
Host species
Rabbit -
Specificity
This antibody detects both phosphorylated (pT538) and unphosphorylated PKC theta/PRKCQ
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Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman -
Immunogen
Synthetic peptide within Human PKC theta/PRKCQ. The exact sequence is proprietary.
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Positive control
- Jurkat cell lysate
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General notes
This product was previously labelled as PKC theta
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1488Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494) at 1/1000 dilution (purified)
Lane 1 : Rat brain lysate
Lane 2 : Rat spleen lysate
Lane 3 : C2C12 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494) at 1/1000 dilution (purified) + Jurkat whole cell lysate at 10 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494) at 1/500 dilution (unpurified) + Jurkat cell lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
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