Anti-PKC theta/PRKCQ (phospho S676) antibody (ab131479)
Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ (phospho S676)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PKC theta/PRKCQ (phospho S676) antibody
See all PKC theta/PRKCQ primary antibodies -
Description
Rabbit polyclonal to PKC theta/PRKCQ (phospho S676) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Human PKC theta/PRKCQ (phospho S676) conjugated to keyhole limpet haemocyanin.
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Positive control
- HeLa cells; PC12 cell extract; Human lung carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131479 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PKC theta/PRKCQ (phospho S676) antibody (ab131479)All lanes : Anti-PKC theta/PRKCQ (phospho S676) antibody (ab131479) at 1/500 dilution
Lane 1 : PC12 cell extract
Lane 2 : PC12 cell extract treated with PMA
Predicted band size: 82 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ (phospho S676) antibody (ab131479)Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling PKC theta/PRKCQ (phospho S676) with ab131479 at 1/50 dilution (left) or ab131479 preincubated with blocking peptide (right).
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Immunocytochemistry/ Immunofluorescence - Anti-PKC theta/PRKCQ (phospho S676) antibody (ab131479)Immunofluorescent analysis of methanol-fixed HeLa cells labelling PKC theta/PRKCQ (phospho S676) with ab131479 at 1/100 dilution.
