Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)
![Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)](https://www.abcam.com/ps/products/278/ab278608/Images/ab278608-2-pkcat497f1k562.jpg "Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [PKCaT497-F1] to PKC alpha (phospho T497)
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1]
See all PKC alpha primary antibodies -
Description
Rabbit monoclonal [PKCaT497-F1] to PKC alpha (phospho T497) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human PKC alpha (phospho T497). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P17252 -
Positive control
- WB: HT1080 (treated with TPA) cell extract; C6 (treated with aniosmycin) cell extract. Flow cyt: K562 cells (treated with EGF + pervanadate); NIH/3T3 cells (treated with pervanadate).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
PKCaT497-F1 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with EGF + pervanadate (green) using ab278608 at 0.1 µg/mL.
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![Western blot - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)](https://www.abcam.com/ps/products/278/ab278608/Images/ab278608-4-ab278608comparisonwb.jpg)
Western blot - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)All lanes : Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608) at 0.05 µg/ml
Lane 1 : HT1080 cell extract treated with TPA
Lane 2 : HT1080 cell extract untreated
Predicted band size: 76 kDa
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![Western blot - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)](https://www.abcam.com/ps/products/278/ab278608/Images/ab278608-6-pkcat497f1c6wb.jpg)
Western blot - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608) at 0.1 µg/ml + C6 cell extract treated with Anisomycin
Predicted band size: 76 kDa
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![Flow Cytometry - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)](https://www.abcam.com/ps/products/278/ab278608/Images/ab278608-5-pkcat497f13t3.jpg)
Flow Cytometry - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)Flow cytometric analysis of 3T3 cells, secondary antibody only negative control (blue) or treated with imatinib (grey) or with pervanadate (orange) using 0.1 µg/mL isotype control, or imatinib (red) or pervanadate (green) using ab278608 at 0.1µg/mL. -
![Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)](https://www.abcam.com/ps/products/278/ab278608/Images/ab278608-1-benefits-of-recombinant-antibodies.png)
Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)