Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [PKCaT497-F1] to PKC alpha (phospho T497)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1]
See all PKC alpha primary antibodies
Description

Rabbit monoclonal [PKCaT497-F1] to PKC alpha (phospho T497)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human PKC alpha (phospho T497). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P17252

Run BLAST with Run BLAST with
Positive control
- WB: HT1080 (treated with TPA) cell extract; C6 (treated with aniosmycin) cell extract. Flow cyt: K562 cells (treated with EGF + pervanadate); NIH/3T3 cells (treated with pervanadate).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

PKCaT497-F1
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or untreated (red) or treated with EGF + pervanadate (green) using ab278608 at 0.1 µg/mL.
Western blot - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)

All lanes : Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608) at 0.05 µg/ml

Lane 1 : HT1080 cell extract treated with TPA
Lane 2 : HT1080 cell extract untreated

Predicted band size: 76 kDa
Western blot - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)

Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608) at 0.1 µg/ml + C6 cell extract treated with Anisomycin

Predicted band size: 76 kDa
Flow Cytometry - Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)

Flow cytometric analysis of 3T3 cells, secondary antibody only negative control (blue) or treated with imatinib (grey) or with pervanadate (orange) using 0.1 µg/mL isotype control, or imatinib (red) or pervanadate (green) using ab278608 at 0.1µg/mL.
Anti-PKC alpha (phospho T497) antibody [PKCaT497-F1] (ab278608)

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