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Cancer Cell cycle Cell differentiation

Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)

Price and availability

526 012 ₸

Availability

Order now and get it on Friday March 19, 2021

Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13440] to IER3IP1 - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, Flow Cyt, WB
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free
    See all IER3IP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13440] to IER3IP1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250460 is the carrier-free version of ab181247. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250460 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13440
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Images

  • Western blot - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    Western blot - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    All lanes : Anti-IER3IP1 antibody [EPR13440] (ab181247) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : HeLa lysate
    Lane 3 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 9 kDa



    This data was developed using ab181247, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    Immunocytochemistry - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    This data was developed using ab181247, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling IER3IP1 with ab181247 at 1/250 dilution, dollowed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and DAPI staining (blue).
  • Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    This data was developed using ab181247, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181247 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000.
  • Flow Cytometry - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    Flow Cytometry - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    This data was developed using ab181247, the same antibody clone in a different buffer formulation.Flow Cytometricl analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling IER3IP1 with ab181247 at 1/210 (red) compared to a negative control antibody (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    This data was developed using ab181247, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling IER3IP1 with ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    This data was developed using ab181247, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IER3IP1 with ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)
    Anti-IER3IP1 antibody [EPR13440] - BSA and Azide free (ab250460)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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