Human BMI1 ELISA Kit (ab272210)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 34 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BMI1 ELISA Kit
See all Bmi1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 1.3% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
34 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 114 112% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human BMI1 ELISA kit (ab272210) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BMI1 protein in cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BMI1 with 34 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
BMI1 (Polycomb Complex Protein BMI-1), also known as Polycomb group RING Finger Protein 4, is a component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, BMI1 regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2. Human BMI1 amino acids sequence is 97% identical to mouse BMI1 and is 95% identical to rat BMI1.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BMI1 Capture Antibody 1 x 600µl 10X Human BMI1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human BMI1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. -
Sequence similarities
Contains 1 RING-type zinc finger. -
Post-translational
modificationsMonoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Alternative names
- B lymphoma Mo MLV insertion region (mouse)
- B lymphoma Mo MLV insertion region 1 homolog
- Bmi 1
see all -
Database links
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Omim: 164831 Human
- SwissProt: P35226 Human
- Unigene: 380403 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human BMI1 standard curve in 1X Cell Extraction Buffer PTR.The BMI1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native BMI1 in human HeLa and K562 based on 300 µg/mL and 150 µg/mL extract loads, respectively.The concentrations of BMI1 were measured in duplicate and interpolated from the BMI1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BMI1 concentration was determined to be 5,142 pg/mL in HeLa extract and 3,399 pg/mL in K562 extract.
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Species reactivity was determined by measuring 100 ug/mL extract of human HeLa, mouse NIH-3T3 and rat PC12 cell extracts and interoplating the protein concentrations from the Human standard curve.The mean BMI1 concentration was determined to be 16.9 ng/mg in human HeLa extract, 12.8 ng/mg in mouse NIH-3T3 extract and 1.2 ng/mg in rat PC12 extract.
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Sandwich ELISA - Human BMI1 ELISA Kit (ab272210)To learn more about the advantages of recombinant antibodies see here.
