Anti-BRCA1 antibody (ab238983)
Key features and details
- Rabbit polyclonal to BRCA1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BRCA1 antibody
See all BRCA1 primary antibodies -
Description
Rabbit polyclonal to BRCA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human BRCA1 aa 979-993.
Database link: P38398 -
Positive control
- WB: MCF7, HeLa and NIH/3T3 cell extracts; Mouse lung extract. IHC-P: Human breast cancer tissue; Mouse liver, testis, spinal cord, brain and pancreas tissue; Rat testis, ovary, pancreas and brain tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRCA1 antibody (ab238983)All lanes : Anti-BRCA1 antibody (ab238983) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) cell extract
Lane 4 : Mouse lung tissue extract
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 207 kDaBlocking and dilution buffer: 3% nonfat dry milk in TBST.
Exposure time: 1 second.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)Paraffin-embedded human breast cancer tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)Paraffin-embedded rat testis tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)Paraffin-embedded rat ovary tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)Paraffin-embedded rat brain tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)Paraffin-embedded rat pancreas tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)Paraffin-embedded mouse liver tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)Paraffin-embedded mouse testis tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)Paraffin-embedded mouse spinal cord tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)Paraffin-embedded mouse brain tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCA1 antibody (ab238983)Paraffin-embedded mouse pancreas tissue stained for BRCA1 using ab238983 at 1/100 dilution in immunohistochemical analysis.
