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Immunology Innate Immunity Macrophage / Inflamm.

Anti-Pyrin antibody [EPR18675] - BSA and Azide free (ab223132)

Anti-Pyrin antibody [EPR18675] - BSA and Azide free (ab223132)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18675] to Pyrin - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-Pyrin antibody [EPR18675] - BSA and Azide free
    See all Pyrin primary antibodies
  • Description

    Rabbit monoclonal [EPR18675] to Pyrin - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: DC2.4 stable Pyrin expression whole cell lysate; Bone marrow derived-macrophage of wild type C57/B6 mice, untreated or stimulated with LPS. IP: DC2.4 stable Pyrin expression whole cell lysate.
  • General notes

    Ab223132 is the carrier-free version of ab214772. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223132 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18675
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins

Images

  • Immunoprecipitation - Anti-Pyrin antibody [EPR18675] - BSA and Azide free (ab223132)
    Immunoprecipitation - Anti-Pyrin antibody [EPR18675] - BSA and Azide free (ab223132)

    Pyrin was immunoprecipitated from 0.35 mg of DC2.4 (Mouse immature dendritic cell line) stable mPyrin expression whole cell lysate with ab214772 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214772 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1:  ab214772 whole cell lysate from DC2.4 stable mPyrin expression cells 10µg (Input).

    Lane 2: ab214772 IP in whole cell lysate from DC2.4 stable mPyrin expression cells.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab214772 in whole cell lysate from DC2.4 stable mPyrin expression cells.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214772).

  • Western blot - Anti-Pyrin antibody [EPR18675] - BSA and Azide free (ab223132)
    Western blot - Anti-Pyrin antibody [EPR18675] - BSA and Azide free (ab223132)
    All lanes : Anti-Pyrin antibody [EPR18675] (ab214772) at 1/1000 dilution

    Lane 1 : Bone marrow derived-macrophage of Pyrin -/- mice
    Lane 2 : Bone marrow derived-macrophage of Pyrin -/- mice stimulated with LPS
    Lane 3 : Bone marrow derived-macrophage of wild type C57/B6 mice
    Lane 4 : Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with LPS

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214772).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The lysate is kindly provided by our collaborator, Feng Shao, National institute of biological sciences, Beijing.

  • Anti-Pyrin antibody [EPR18675] - BSA and Azide free (ab223132)
    Anti-Pyrin antibody [EPR18675] - BSA and Azide free (ab223132)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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