Cyclooxygenase 1 (COX1) Inhibitor Assay Kit (Fluorometric) (ab204698)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
Cyclooxygenase 1 (COX1) Inhibitor Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity -
Product overview
Cyclooxygenase 1 (COX1) Inhibitor Screening Kit (Fluorometric) (ab204698) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of COX-1 inhibitors. The assay is based on the fluorometric detection of Prostaglandin G2, the intermediate product generated by the COX enzyme.
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Notes
Cyclooxygenase (COX), also known as prostaglandin-endoperoxide synthase (PTGS, EC 1.14.99.1), is an enzyme that is responsible for the formation of important biological mediators called prostanoids, including prostaglandins, prostacyclin and thromboxane. COX is the central enzyme in the biosynthetic pathway to prostanoids from arachidonic acid. There are two known isoenzymes: COX-1 and COX-2. COX-1 is constitutively expressed in many tissues and is the predominant form in gastric mucosa and in kidney. COX-2 is not expressed under normal conditions in most cells, but elevated levels are found during inflammation. Pharmacological inhibition of COX by nonsteroidal anti-inflammatory drugs (NSAID) can provide relief from the symptoms of inflammation and pain.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Arachidonic Acid 1 x 50µl COX Assay Buffer 1 x 25ml COX Cofactor (in DMSO) 1 x 20µl COX Probe (in DMSO) 1 x 200µl COX-1, Ovine 1 vial NaOH 1 x 500µl SC560, COX-1 inhibitor (in DMSO) 1 x 100µl -
Research areas
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Relevance
COX proteins are membrane-associated heme proteins that have cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (nonsteroidal anti-inflammatory drugs) such as aspirin. Prostaglandins (PGs) formed by the enzymatic activity of COX-1 are primarily involved in the regulation of homeostatic functions throughout the body, whereas PGs formed by COX-2 primarily mediate pain, fever, and inflammation. COX-1 is constitutively expressed, with particularly high expression in gastrointestinal tissues. COX-2 is induced by cytokines and mitogens and is likely to play a role in inflammatory diseases such as rheumatoid arthritis. In rodents and humans, COX-3 encodes proteins with completely different amino acid sequences from COX-1 or COX-2, and without COX activity. COX-3 is thought to be a splice variant of COX-1 which retains intron one. It has been suggested that COX-3 may be the key to unlocking the mechanism of action of acetaminophen. -
Cellular localization
Membrane-associated; Microsomal membrane. -
Alternative names
- Cox3
- Cyclooxygenase 1
- Ptgs1
Images
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Cyclooxygenase 1 (COX1) Inhibitor Screening Kit (Fluorometric) (ab204698)
Inhibition of Cyclooxygenase 1 (COX1) Activity with SC560. IC50 of SC560 was determined to be 6.45 nM.
