3-Carboxyumbelliferyl ß-D-galactoside, BSA conjugate (ab275363)
Key features and details
- Detection of extracellular β-galactosidase activity in enzyme-linked antibody systems or for endocytosis experiments, measurement of lacY permease activity, or microinjection analyses.
- Purity: > 98%
- Soluble in water.
- Form / State: Solid
- Source: Synthetic
Overview
-
Product name
3-Carboxyumbelliferyl ß-D-galactoside, BSA conjugate -
Description
Detection of extracellular β-galactosidase activity in enzyme-linked antibody systems or for endocytosis experiments, measurement of lacY permease activity, or microinjection analyses. -
Biological description
A conjugate of BSA (bovine serum albumin, MW = 66.4K) and the beta-galactosidase substrate carboxyumbelliferyl beta-D-galactopyranoside ab275363 produces a protein that can be used to measure extracellular β-galactosidase activity, measure lacY permease activity, for microinjection analyses, or measure cell disruption or lysis.
-
Purity
> 98% -
General notes
The fluorescence spectrum for 3-carboxyumbelliferone is pH-dependent. Below the pKa (7.8), Abs shifts to shorter wavelengths (325-340 nm) and fluorescence intensity decreases. These shifts may be used to measure compartmentalization of intracellular activity.
-
Chemical structure
Properties
-
Molecular weight
66800.00 -
Storage instructions
Shipped at 4°C. Store at -20°C. Store under desiccating conditions. -
Solubility overview
Soluble in water. -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
Source
Synthetic
Images
-
Chemical Structure - 3-Carboxyumbelliferyl ß-D-galactoside, BSA conjugate (ab275363)
Chemical structure of 3-Carboxyumbelliferyl beta-D-galactoside, BSA conjugate