Anti-Pyrin (phospho S241) antibody [EPR19570] (ab200420)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19570] to Pyrin (phospho S241)
- Suitable for: WB, Dot blot
- Reacts with: Mouse
Overview
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Product name
Anti-Pyrin (phospho S241) antibody [EPR19570]
See all Pyrin primary antibodies -
Description
Rabbit monoclonal [EPR19570] to Pyrin (phospho S241) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Cell lysate of DC2.4 expressing Pyrin.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19570 -
Isotype
IgG -
Research areas
Images
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Dot blot analysis of Pyrin (phospho S241) labeled with ab200420 at 1/1000 dilution.
Lane 1: Pyrin (phospho S241) phospho peptide;
Lane 2: Pyrin Non-phospho peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-Pyrin (phospho S241) antibody [EPR19570] (ab200420) at 1/1000 dilution
Lane 1 : Cell lysate of DC2.4 (Mouse immature dendritic cell line) expressing mPyrin
Lane 2 : Cell lysate of DC2.4 (Mouse immature dendritic cell line) expressing mPyrin. mPyrin dephosphorylation is stimulated with TcdA.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
The lysates were kindly provided by our collaborator Feng Shao, National institute of biological sciences Beijing, China.
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