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Epigenetics and Nuclear Signaling Histones H3 Methylated

Anti-Histone H3 (di methyl K14) antibody [RM165] (ab227874)

Anti-Histone H3 (di methyl K14) antibody [RM165] (ab227874)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [RM165] to Histone H3 (di methyl K14)
  • Suitable for: WB, Multiplex Protein Detection
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-Histone H3 (di methyl K14) antibody [RM165]
    See all Histone H3 primary antibodies
  • Description

    Rabbit monoclonal [RM165] to Histone H3 (di methyl K14)
  • Host species

    Rabbit
  • Specificity

    ab227874 reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with monomethylated Lysine 14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in histone H3.
  • Tested applications

    Suitable for: WB, Multiplex Protein Detectionmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Synthetic peptide corresponding to Human Histone H3 (di methyl K14).
    Database link: P68431

  • Positive control

    • WB: Acid extracts of HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM165
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated

Images

  • Western blot - Anti-Histone H3 (di methyl K14) antibody [RM165] (ab227874)
    Western blot - Anti-Histone H3 (di methyl K14) antibody [RM165] (ab227874)
    All lanes : Anti-Histone H3 (di methyl K14) antibody [RM165] (ab227874) at 0.25 µg/ml

    Lane 1 : Recombinant Histone H3.3
    Lane 2 : Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells

    Predicted band size: 15 kDa

  • Multiplex Protein Detection - Anti-Histone H3 (di methyl K14) antibody [RM165] (ab227874)
    Multiplex Protein Detection - Anti-Histone H3 (di methyl K14) antibody [RM165] (ab227874)

    ab227874 specifically reacts to Histone H3 dimethylated at Lysine 14 (K14me2).

    No cross reactivity with non-modified Lysine 14 (H3K14), monomethylated Lysine14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in Histone H3.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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