Anti-Histone H3 (di methyl K14) antibody [RM165] (ab227874)
Key features and details
- Rabbit monoclonal [RM165] to Histone H3 (di methyl K14)
- Suitable for: WB, Multiplex Protein Detection
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-Histone H3 (di methyl K14) antibody [RM165]
See all Histone H3 primary antibodies -
Description
Rabbit monoclonal [RM165] to Histone H3 (di methyl K14) -
Host species
Rabbit -
Specificity
ab227874 reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with monomethylated Lysine 14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in histone H3. -
Tested applications
Suitable for: WB, Multiplex Protein Detectionmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (di methyl K14).
Database link: P68431 -
Positive control
- WB: Acid extracts of HeLa cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM165 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-Histone H3 (di methyl K14) antibody [RM165] (ab227874) at 0.25 µg/ml
Lane 1 : Recombinant Histone H3.3
Lane 2 : Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells
Predicted band size: 15 kDa
-
ab227874 specifically reacts to Histone H3 dimethylated at Lysine 14 (K14me2).
No cross reactivity with non-modified Lysine 14 (H3K14), monomethylated Lysine14 (K14me1), trimethylated Lysine 14 (K14me3), or other methylations in Histone H3.