Anti-Pyrin antibody [EPR18676] - BSA and Azide free (ab222484)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18676] to Pyrin - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse
Overview
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Product name
Anti-Pyrin antibody [EPR18676] - BSA and Azide free
See all Pyrin primary antibodies -
Description
Rabbit monoclonal [EPR18676] to Pyrin - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Bone marrow derived-macrophage of wild type C57/B6 mice, untreated and stimulated with LPS or TNF alpha; DC2.4 stable expression whole cell lysate. IP: DC2.4 stable expression whole cell lysate.
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General notes
Ab222484 is the carrier-free version of ab195975. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab222484 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18676 -
Isotype
IgG -
Research areas
Images
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Pyrin was immunoprecipitated from DC2.4 (Human immature dendritic cell line) stable Pyrin expression whole cell lysate with ab195975 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab195975 at 1/1000 dilution. Goat anti-Rabbit IgG (H+L), was used as secondary antibody at 1/5000 dilution.
Lane 1: DC2.4 whole cell lysate.
Lane 2: DC2.4 stable Pyrin expression whole cell lysate.
Lane 3: DC2.4 stable Pyrin expression whole cell lysate.
Lane 4: ab195975 IP in DC2.4 whole cell lysate.
Lane 5: ab195975 IP in DC2.4 stable Pyrin expression whole cell lysate.
Lane 6: Mock IP (without ab195975) in DC2.4 stable Pyrin expression whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195975).
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All lanes : Anti-Pyrin antibody [EPR18676] (ab195975) at 1/1000 dilution
Lane 1 : Bone marrow derived-macrophage of Pyrin -/- mice
Lane 2 : Bone marrow derived-macrophage of Pyrin -/- mice stimulated with LPS
Lane 3 : Bone marrow derived-macrophage of Pyrin -/- mice stimulated with TNF alpha
Lane 4 : Bone marrow derived-macrophage of wild type C57/B6 mice
Lane 5 : Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with LPS
Lane 6 : Bone marrow derived-macrophage of wild type C57/B6 mice stimulated with TNF alpha
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 86 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195975).
Blocking/Dilution buffer: 5% milk/TBST.
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