Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)

Key features and details

  • Rabbit polyclonal to Zinc Alpha 2 Glycoprotein
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Zinc Alpha 2 Glycoprotein antibody
    See all Zinc Alpha 2 Glycoprotein primary antibodies

  • Description

    Rabbit polyclonal to Zinc Alpha 2 Glycoprotein

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Liver; Human Plasma Total Protein; Human Heart; Human Lung; Human Adipose. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: HeLa.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.

  • Tissue specificity

    Blood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver.

  • Sequence similarities

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Alpha 2 glycoprotein 1 zinc antibody
    • Alpha 2 glycoprotein 1 zinc binding antibody
    • Alpha 2 glycoprotein zinc antibody
    • Azgp1 antibody
    • ZA2G antibody
    • ZA2G_HUMAN antibody
    • ZAG antibody
    • Zinc alpha 2 glycoprotein precursor antibody
    • Zinc-alpha-2-glycoprotein antibody
    • Zn alpha 2 glycoprotein antibody
    • Zn alpha 2 GP antibody
    • Zn alpha2 glycoprotein antibody
    • Zn-alpha-2-glycoprotein antibody
    • Zn-alpha-2-GP antibody
    • ZNGP1 antibody
    see all

Images

  • Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
    Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
    All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human Plasma Total Protein Lysate
    Lane 3 : Human heart tissue lysate - total protein (ab29431)
    Lane 4 : Lung (Human) Tissue Lysate
    Lane 5 : Human adipose normal tissue lysate - total protein (ab28980)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    Zinc Alpha 2 Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunocytochemistry/ Immunofluorescence - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
    Immunocytochemistry/ Immunofluorescence - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)

    ICC/IF image of ab117275 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab117275 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
    IHC image of ab117275 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117275, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References (7)

Publishing research using ab117275? Please let us know so that we can cite the reference in this datasheet.

ab117275 has been referenced in 7 publications.

  • Guo J  et al. Adipokine zinc-a2-glycoprotein alleviates lipopolysaccharide-induced inflammatory responses through the ß3-AR/PKA/CREB pathway. Cytokine 123:154742 (2019). PubMed: 31260855
  • Liu J  et al. AZGP1 inhibits soft tissue sarcoma cells invasion and migration. BMC Cancer 18:89 (2018). WB, IHC-P ; Human . PubMed: 29357838
  • Gao SX  et al. ZAG alleviates HFD-induced insulin resistance accompanied with decreased lipid depot in skeletal muscle in mice. J Lipid Res 59:2277-2286 (2018). PubMed: 30318474
  • Philley JV  et al. Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease. Oncotarget 8:18070-18081 (2017). PubMed: 28160560
  • Xue Y  et al. Zinc-a-2-glycoprotein: a candidate biomarker for colon cancer diagnosis in Chinese population. Int J Mol Sci 16:691-703 (2015). IHC ; Human . PubMed: 25561225
  • Priyadarsini S  et al. Gross cystic disease fluid protein-15/prolactin-inducible protein as a biomarker for keratoconus disease. PLoS One 9:e113310 (2014). WB ; Human . PubMed: 25405607
  • Yang MH  et al. Proteomic profiling for peritoneal dialysate: differential protein expression in diabetes mellitus. Biomed Res Int 2013:642964 (2013). PubMed: 23781506

Images

  • Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
    Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
    All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human Plasma Total Protein Lysate
    Lane 3 : Human heart tissue lysate - total protein (ab29431)
    Lane 4 : Lung (Human) Tissue Lysate
    Lane 5 : Human adipose normal tissue lysate - total protein (ab28980)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    Zinc Alpha 2 Glycoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Immunocytochemistry/ Immunofluorescence - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
    Immunocytochemistry/ Immunofluorescence - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)

    ICC/IF image of ab117275 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab117275 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody (ab117275)
    IHC image of ab117275 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117275, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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