Anti-MASP1 antibody [EPR22680-36] - BSA and Azide free (ab256814)
![Anti-MASP1 antibody [EPR22680-36] - BSA and Azide free (ab256814)](https://www.abcam.com/ps/products/256/ab256814/Images/ab256814-352421-antimasp1antibodyepr2268036immunocytochemistryraw2647mouse.jpg "Anti-MASP1 antibody [EPR22680-36] - BSA and Azide free (ab256814)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22680-36] to MASP1 - BSA and Azide free
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat
Overview
-
Product name
Anti-MASP1 antibody [EPR22680-36] - BSA and Azide free
See all MASP1 primary antibodies -
Description
Rabbit monoclonal [EPR22680-36] to MASP1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Rat liver, serum, lung and plasma lysate; mouse plasma, liver and serum lysate. ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells.
-
General notes
Ab256814 is the carrier-free version of ab255814. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256814 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22680-36 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22680-36] - BSA and Azide free (ab256814)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling MASP1 with ab255814 at a 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at a 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cells is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255814).
-
![Flow Cytometry - Anti-MASP1 antibody [EPR22680-36] - BSA and Azide free (ab256814)](https://www.abcam.com/ps/products/256/ab256814/Images/ab256814-1-anti-masp1-antibody-epr22680-36-flowcytometry-raw-2647-mouse.jpg)
Flow Cytometry - Anti-MASP1 antibody [EPR22680-36] - BSA and Azide free (ab256814)Flow cytometric analysis of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling MASP1 with ab255814 at a 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255814).
-
![Anti-MASP1 antibody [EPR22680-36] - BSA and Azide free (ab256814)](https://www.abcam.com/ps/products/256/ab256814/Images/ab256814-3-benefits-of-recombinant-antibodies.png)
Anti-MASP1 antibody [EPR22680-36] - BSA and Azide free (ab256814)
