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Human MAP3K11 (MLK3) knockout HeLa cell pellet (ab279075)

Overview

  • Product name

    Human MAP3K11 (MLK3) knockout HeLa cell pellet
    See all MLK3 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and 1 bp deletion in exon 1.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.

  • Tissue specificity

    Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.

  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.

  • Post-translational
    modifications

    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.

  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
  • Information by UniProt

  • Alternative names

    • 2610017K16Rik
    • EC 2.7.11.25
    • M3K11_HUMAN
    • Map3k11
    • MEKK11
    • MGC17114
    • Mitogen-activated protein kinase kinase kinase 11
    • Mixed lineage kinase 3
    • Mixed lineage protein kinase 3
    • Mlk-3
    • Mlk3
    • Protein tyrosine kinase PTK1
    • PTK1
    • RHOE
    • SH3 domain containing proline rich kinase
    • SPRK
    • Src-homology 3 domain-containing proline-rich kinase
    see all

Properties

Images

  • Sanger Sequencing - Human MAP3K11 (MLK3) knockout HeLa cell pellet (ab279075)
    Sanger Sequencing - Human MAP3K11 (MLK3) knockout HeLa cell pellet (ab279075)

    Allele-1: 11 bp deletion in exon 1

     

  • Sanger Sequencing - Human MAP3K11 (MLK3) knockout HeLa cell pellet (ab279075)
    Sanger Sequencing - Human MAP3K11 (MLK3) knockout HeLa cell pellet (ab279075)

    Allele-2: 1 bp deletion in exon 1

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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