Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI1A2] to MEK2
Suitable for: WB, ICC/IF, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-MEK2 antibody [OTI1A2]
See all MEK2 primary antibodies
Description

Mouse monoclonal [OTI1A2] to MEK2
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human MEK2 aa 1-400. Produced in HEK-293T cells. NP_109587
Sequence:
MLARRKPVLPALTINPTIAEGPSPTSEGASEANLVDLQKKLEELELDEQQ KKRLEAFLTQKAKVGELKDDDFERISELGAGNGGVVTKVQHRPSGLIMAR KLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHM DGGSLDQVLKEAKRIPEEILGKVSIAVLRGLAYLREKHQIMHRDVKPSNI LVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMAPERLQGTHYSVQSDI WSMGLSLVELAVGRYPIPPPDAKELEAIFGRPVVDGEEGEPHSISPRPRP PGRPVSGHGMDSRPAMAIFELLDYIVNEPPPKLPNGVFTPDFQEFVNKCL IKNPAERADLKMLTNHTFIKRSEVEEVDFAGWLCKTLRLNQPGTPTRTAV

Database link: P36507
Run BLAST with Run BLAST with
Positive control
- WB: HEK-293T cells transfected with pCMV6-ENTRY MEK2 cDNA; HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC-12 and MCF7 cell extracts. IHC-P: Human kidney, liver, liver carcinoma, lung carcinoma, pancreas and endometrium tissues. ICC/IF: pCMV6-ENTRY MEK2 cDNA transfected COS-7 cells.
General notes

The clone number has been updated from 1A2 to OTI1A2, both clone numbers name the same clone.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography
Clonality

Monoclonal
Clone number

OTI1A2
Isotype

IgG1
Research areas

Western blot - Anti-MEK2 antibody [OTI1A2] (ab140372)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MEK2 knockout HAP1 cell lysate (20 µg)

Lane 3: Jurkat cell lysate (20 µg)
Lane 4: K562 knockout HAP1 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab140372 observed at 44 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab140372 was shown to recognize MEK2 when MEK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK2 knockout samples were subjected to SDS-PAGE. ab140372 and ab181602 (loading control to GAPDH) were diluted 1/200 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-MEK2 antibody [OTI1A2] (ab140372)

All lanes : Anti-MEK2 antibody [OTI1A2] (ab140372) at 1/500 dilution

Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells
Lane 2 : MEK2-Knockout HEK-293T cells

Lysates/proteins at 10 µg per lane.

Predicted band size: 44 kDa

The blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [OTI1A2] (ab140372)

Paraffin-embedded human kidney tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Immunocytochemistry/ Immunofluorescence - Anti-MEK2 antibody [OTI1A2] (ab140372)

pCMV6-ENTRY MEK2 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for MEK2 using ab140372 at a 1/100 dilution in ICC/IF.
Western blot - Anti-MEK2 antibody [OTI1A2] (ab140372)

All lanes : Anti-MEK2 antibody [OTI1A2] (ab140372) at 1/200 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 (Mouse cell line) cell extract
Lane 4 : A549 (Human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract

Lysates/proteins at 35 µg per lane.

Predicted band size: 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [OTI1A2] (ab140372)

Paraffin-embedded human liver tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [OTI1A2] (ab140372)

Paraffin-embedded human liver carcinoma tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [OTI1A2] (ab140372)

Paraffin-embedded human lung tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [OTI1A2] (ab140372)

Paraffin-embedded human pancreas tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [OTI1A2] (ab140372)

Paraffin-embedded human endometrium tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.
Western blot - Anti-MEK2 antibody [OTI1A2] (ab140372)

All lanes : Anti-MEK2 antibody [OTI1A2] (ab140372) at 1/200 dilution

Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY MEK2 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 44 kDa

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