Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MEK2 knockout HAP1 cell lysate (20 µg)

Lane 3: Jurkat cell lysate (20 µg)
Lane 4: K562 knockout HAP1 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab140372 observed at 44 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab140372 was shown to recognize MEK2 when MEK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK2 knockout samples were subjected to SDS-PAGE. ab140372 and ab181602 (loading control to GAPDH) were diluted 1/200 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes : Anti-MEK2 antibody [OTI1A2] (ab140372) at 1/500 dilution

Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells
Lane 2 : MEK2-Knockout HEK-293T cells

Lysates/proteins at 10 µg per lane.

Predicted band size: 44 kDa

The blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.

Paraffin-embedded human kidney tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

pCMV6-ENTRY MEK2 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for MEK2 using ab140372 at a 1/100 dilution in ICC/IF.

All lanes : Anti-MEK2 antibody [OTI1A2] (ab140372) at 1/200 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 (Mouse cell line) cell extract
Lane 4 : A549 (Human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract

Lysates/proteins at 35 µg per lane.

Predicted band size: 44 kDa

Paraffin-embedded human liver tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

Paraffin-embedded human liver carcinoma tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

Paraffin-embedded human lung tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

Paraffin-embedded human pancreas tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

Paraffin-embedded human endometrium tissue stained for MEK2 with ab140372 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

All lanes : Anti-MEK2 antibody [OTI1A2] (ab140372) at 1/200 dilution

Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY MEK2 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 44 kDa