Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MASP1 antibody [EPR22680-36] (ab255814)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22680-36] to MASP1
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-MASP1 antibody [EPR22680-36]
See all MASP1 primary antibodies
Description

Rabbit monoclonal [EPR22680-36] to MASP1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat liver, serum, lung and plasma lysate; mouse plasma, liver and serum lysate. ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22680-36
Isotype

IgG
Research areas

Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)

All lanes : Anti-MASP1 antibody [EPR22680-36] (ab255814) at 1/1000 dilution

Lane 1 : Rat liver lysate
Lane 2 : Rat serum lysate
Lane 3 : Rat lung lysate
Lane 4 : Rat plasma lysate
Lane 5 : Mouse plasma lysate
Lane 6 : Mouse serum lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 79 kDa
Observed band size: 90, 28 kDa
why is the actual band size different from the predicted?

The molecular weight observed is consistent with what has been described in the literature (PMID: 24472859, 27232184).

Exposure times: Lanes 1-3: 59 secs; Lane 4: 26 secs; Lanes 5-6: 15 secs.

Blocking/Diluting buffer: 5% NFDM/TBST.
Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)

All lanes : Anti-MASP1 antibody [EPR22680-36] (ab255814) at 1/1000 dilution

Lane 1 : Mouse liver lysate
Lane 2 : Mouse pancreas lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 79 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

The molecular weight observed is consistent with what has been described in the literature (PMID: 24472859, 27232184).

Negative control: Pancreas (based on Expression Atlas, GTEx and HPA databases).

Blocking/Diluting buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22680-36] (ab255814)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling MASP1 with ab255814 at a 1/50 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at a 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cells is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Flow Cytometry - Anti-MASP1 antibody [EPR22680-36] (ab255814)

Flow cytometric analysis of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling MASP1 with ab255814 at a 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at a 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Anti-MASP1 antibody [EPR22680-36] (ab255814)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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