Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MASP1 antibody [EPR22680-36] (ab255814)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22680-36] to MASP1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MASP1 antibody [EPR22680-36]
    See all MASP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22680-36] to MASP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat liver, serum, lung and plasma lysate; mouse plasma, liver and serum lysate. ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22680-36

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)
    Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)
    All lanes : Anti-MASP1 antibody [EPR22680-36] (ab255814) at 1/1000 dilution

    Lane 1 : Rat liver lysate
    Lane 2 : Rat serum lysate
    Lane 3 : Rat lung lysate
    Lane 4 : Rat plasma lysate
    Lane 5 : Mouse plasma lysate
    Lane 6 : Mouse serum lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 79 kDa
    Observed band size: 90, 28 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 24472859, 27232184).

    Exposure times: Lanes 1-3: 59 secs; Lane 4: 26 secs; Lanes 5-6: 15 secs.

    Blocking/Diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)
    Western blot - Anti-MASP1 antibody [EPR22680-36] (ab255814)
    All lanes : Anti-MASP1 antibody [EPR22680-36] (ab255814) at 1/1000 dilution

    Lane 1 : Mouse liver lysate
    Lane 2 : Mouse pancreas lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 79 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    The molecular weight observed is consistent with what has been described in the literature (PMID: 24472859, 27232184).

    Negative control: Pancreas (based on Expression Atlas, GTEx and HPA databases).

    Blocking/Diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22680-36] (ab255814)
    Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22680-36] (ab255814)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling MASP1 with ab255814 at a 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at a 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cells is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Flow Cytometry - Anti-MASP1 antibody [EPR22680-36] (ab255814)
    Flow Cytometry - Anti-MASP1 antibody [EPR22680-36] (ab255814)

    Flow cytometric analysis of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling MASP1 with ab255814 at a 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Anti-MASP1 antibody [EPR22680-36] (ab255814)
    Anti-MASP1 antibody [EPR22680-36] (ab255814)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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