Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GLO1 antibody [EPR8878] (ab137098)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8878] to GLO1
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-GLO1 antibody [EPR8878]
    See all GLO1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8878] to GLO1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GLO1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Molt-4, Raji, TF-1, HeLa, and red blood cell lysates; Molt-4 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
    Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
    All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : GLO1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 21 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab137098 observed at 21 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab137098 was shown to specifically react with in wild-type HAP1 cells as signal was lost in GLO1 knockout cells. Wild-type and GLO1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab137098 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
    Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
    All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

    Lane 1 : Molt-4 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : TF-1 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Red blood cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 21 kDa

  • Flow Cytometry - Anti-GLO1 antibody [EPR8878] (ab137098)
    Flow Cytometry - Anti-GLO1 antibody [EPR8878] (ab137098)
    Flow cytometric analysis of permeabilized Molt-4 cells labelling GLO1 with ab137098 at 1/10 dilution (red). Negative Rabbit IgG shown in green.
  • Anti-GLO1 antibody [EPR8878] (ab137098)
    Anti-GLO1 antibody [EPR8878] (ab137098)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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