Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8878] to GLO1
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-GLO1 antibody [EPR8878]
See all GLO1 primary antibodies
Description

Rabbit monoclonal [EPR8878] to GLO1
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human GLO1 aa 1-100. The exact sequence is proprietary.
Positive control
- Molt-4, Raji, TF-1, HeLa, and red blood cell lysates; Molt-4 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8878
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- Other

Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)

All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GLO1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 21 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab137098 observed at 21 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab137098 was shown to specifically react with in wild-type HAP1 cells as signal was lost in GLO1 knockout cells. Wild-type and GLO1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab137098 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)

All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

Lane 1 : Molt-4 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : TF-1 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Red blood cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 21 kDa
Flow Cytometry - Anti-GLO1 antibody [EPR8878] (ab137098)

Flow cytometric analysis of permeabilized Molt-4 cells labelling GLO1 with ab137098 at 1/10 dilution (red). Negative Rabbit IgG shown in green.
Anti-GLO1 antibody [EPR8878] (ab137098)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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