Anti-Lumican antibody [EPR8898(2)] (ab168348)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8898(2)] to Lumican
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Lumican antibody [EPR8898(2)]
See all Lumican primary antibodies -
Description
Rabbit monoclonal [EPR8898(2)] to Lumican -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Lumican aa 250-350. The exact sequence is proprietary.
Database link: P51884 -
Positive control
- WB: Saos-2, fetal skin, fetal heart, Jurkat, Y79 and HEK-293T lysates. IHC-P: Human kidney and Human skin tissues. ICC/IF: Saos-2 cells. Flow cyt: HEK-293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8898(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Lumican antibody [EPR8898(2)] (ab168348) at 1/1000 dilution
Lane 1 : Saos-2 lysate
Lane 2 : Fetal skin lysate
Lane 3 : Fetal heart lysate
Lane 4 : Jurkat lysate
Lane 5 : Y79 lysate
Lane 6 : 293T lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Lumican with ab168348 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of Saos-2 cells labeling Lumican with ab168348 at 1/250.
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Flow Cytometrical analysis of permeabilized 293T cells with ab168348 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Lumican with ab168348 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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