Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAC antibody [EPR12805] - N-terminal (ab181157)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12805] to STAC - N-terminal
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-STAC antibody [EPR12805] - N-terminal
    See all STAC primary antibodies

  • Description

    Rabbit monoclonal [EPR12805] to STAC - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • U87-MG, 293, Human fetal brain or A431 lysate, A431 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12805

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)
    Immunocytochemistry/ Immunofluorescence - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)

    Immunofluorescence analysis of A431 cells (fixative 4% paraformaldehyde) labeling STAC with ab181157 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution. Counter stained with Dapi (blue).

  • Flow Cytometry - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)
    Flow Cytometry - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)

    Flow cytometry analysis of A431 cells labeling STAC using ab181157 at a 1/20 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Western blot - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)
    Western blot - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)
    All lanes : Anti-STAC antibody [EPR12805] - N-terminal (ab181157) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : A431 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

  • Western blot - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)
    Western blot - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)
    All lanes : Anti-STAC antibody [EPR12805] - N-terminal (ab181157) at 1/10000 dilution

    Lane 1 : U87-MG lysate
    Lane 2 : 293 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa

  • Anti-STAC antibody [EPR12805] - N-terminal (ab181157)
    Anti-STAC antibody [EPR12805] - N-terminal (ab181157)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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