Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Tuesday March 02, 2021

Anti-STAC antibody [EPR12805] - N-terminal (ab181157)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12805] to STAC - N-terminal
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-STAC antibody [EPR12805] - N-terminal
See all STAC primary antibodies
Description

Rabbit monoclonal [EPR12805] to STAC - N-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- U87-MG, 293, Human fetal brain or A431 lysate, A431 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12805
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)

Immunofluorescence analysis of A431 cells (fixative 4% paraformaldehyde) labeling STAC with ab181157 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution. Counter stained with Dapi (blue).
Flow Cytometry - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)

Flow cytometry analysis of A431 cells labeling STAC using ab181157 at a 1/20 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
Western blot - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)

All lanes : Anti-STAC antibody [EPR12805] - N-terminal (ab181157) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : A431 lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa
Western blot - Anti-STAC antibody [EPR12805] - N-terminal (ab181157)

All lanes : Anti-STAC antibody [EPR12805] - N-terminal (ab181157) at 1/10000 dilution

Lane 1 : U87-MG lysate
Lane 2 : 293 lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa
Anti-STAC antibody [EPR12805] - N-terminal (ab181157)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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