Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MASP1 antibody [EPR22804-163] (ab255731)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22804-163] to MASP1
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-MASP1 antibody [EPR22804-163]
    See all MASP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22804-163] to MASP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma; HepG2 and A549 whole cell lysates. ICC/IF: A549 cells. Flow cyt: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22804-163

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22804-163] (ab255731)
    Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22804-163] (ab255731)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling MASP1 with ab255731 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cells is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1:1000 dilution.

  • Flow Cytometry - Anti-MASP1 antibody [EPR22804-163] (ab255731)
    Flow Cytometry - Anti-MASP1 antibody [EPR22804-163] (ab255731)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol  permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling MASP1 with ab255731 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.  

  • Western blot - Anti-MASP1 antibody [EPR22804-163] (ab255731)
    Western blot - Anti-MASP1 antibody [EPR22804-163] (ab255731)
    All lanes : Anti-MASP1 antibody [EPR22804-163] (ab255731) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : A549 (human lung carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 79 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 24472859, 27232184).

  • Western blot - Anti-MASP1 antibody [EPR22804-163] (ab255731)
    Western blot - Anti-MASP1 antibody [EPR22804-163] (ab255731)
    Anti-MASP1 antibody [EPR22804-163] (ab255731) at 1/1000 dilution + Human plasma at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?


    Exposure time: 37 seconds


    The molecular weight observed is consistent with what has been described in the literature (PMID: 24472859, 27232184).

    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

  • Anti-MASP1 antibody [EPR22804-163] (ab255731)
    Anti-MASP1 antibody [EPR22804-163] (ab255731)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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