Anti-PLK1 antibody (ab70695)
Key features and details
- Rabbit polyclonal to PLK1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLK1 antibody
See all PLK1 primary antibodies -
Description
Rabbit polyclonal to PLK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human PLK1.
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Positive control
- WB: Hela whole cell lysate; IP: K-562 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-PLK1 antibody (ab70695)
PLK1 was immunoprecipitated from K-562 cells whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded), prepared using NETN lysis buffer. ab70695 was used for IP at 6 µg per reaction. PLK1 was also immunoprecipitated. For blotting immunoprecipitated PLK1, ab70695 was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-PLK1 antibody (ab70695)Lane 1 : Anti-PLK1 antibody (ab70695) at 1 µg/ml
Lane 2 : Anti-PLK1 antibody (ab70695) at 0.5 µg/ml
All lanes : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 68 kDa
Observed band size: 68 kDa
