Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Protein CASP antibody [EPR18806] - BSA and Azide free
See all Protein CASP primary antibodies
Description

Rabbit monoclonal [EPR18806] to Protein CASP - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IP	Human
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab250607 is the carrier-free version of ab182216. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250607 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18806
Isotype

IgG
Research areas

Western blot - Anti-Protein CASP antibody [EPR18806] - BSA and Azide free (ab250607)

All lanes : Anti-Protein CASP antibody [EPR18806] (ab182216) at 1/2000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

Exposure time: 30 seconds

This data was developed using ab182216, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Protein CASP antibody [EPR18806] - BSA and Azide free (ab250607)

All lanes : Anti-Protein CASP antibody [EPR18806] (ab182216) at 1/2000 dilution

Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

This data was developed using ab182216, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-2: 30 seconds; Lane 3: 3 minutes.
Western blot - Anti-Protein CASP antibody [EPR18806] - BSA and Azide free (ab250607)

All lanes : Anti-Protein CASP antibody [EPR18806] (ab182216) at 1/2000 dilution

Lane 1 : Rat testis lysate
Lane 2 : Rat cerebral cortex lysate
Lane 3 : Mouse cerebral cortex lysate
Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 5 : Mouse testis lysate
Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 7 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

This data was developed using ab182216, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: Lanes 1-4: 3 minutes; Lanes 5-7: 30 seconds.
Western blot - Anti-Protein CASP antibody [EPR18806] - BSA and Azide free (ab250607)

All lanes : Anti-Protein CASP antibody [EPR18806] (ab182216) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lane 6 : Rat spleen lysate
Lane 7 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 8 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

This data was developed using ab182216, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-6: 30 seconds; Lanes 7-10: 8 seconds.
Immunocytochemistry - Anti-Protein CASP antibody [EPR18806] - BSA and Azide free (ab250607)

This data was developed using ab182216, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Protein CASP with ab182216 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific but weak cytoplasmic staining on U-87 MG cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor^®594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows: -ve control 1: ab210528 at 1/100 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Flow Cytometry - Anti-Protein CASP antibody [EPR18806] - BSA and Azide free (ab250607)

This data was developed using ab182216, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Protein CASP with ab182216 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor^® 488) at 1/500 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Protein CASP antibody [EPR18806] - BSA and Azide free (ab250607)

This data was developed using ab182216, the same antibody clone in a different buffer formulation.Protein CASP was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab182216 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab182216 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HepG2 whole cell lysate, 10µg (Input). Lane 2: ab182216 IP in HepG2 whole cell lysate. Lane 3: Rabbit IgG, [EPR25A] - Isotype Control (ab172730) instead of ab182216 in HepG2 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds.
Immunocytochemistry - Anti-Protein CASP antibody [EPR18806] - BSA and Azide free (ab250607)

This data was developed using ab182216, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Protein CASP with ab182216 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific but weak cytoplasmic staining on NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor^®594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows: -ve control 1: ab210528 at 1/100 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Flow Cytometry - Anti-Protein CASP antibody [EPR18806] - BSA and Azide free (ab250607)

This data was developed using ab182216, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Protein CASP with ab182216 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] -Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor^® 488) at 1/500 dilution was used as the secondary antibody. Note: Cells were permeabilized with 90% methanol (in 1XPBS).
Anti-Protein CASP antibody [EPR18806] - BSA and Azide free (ab250607)