Mouse Pin1 Matched Antibody Pair Kit (ab218178)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Mouse Pin1 Matched Antibody Pair Kit
See all Pin1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
4.6 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Pin1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Pin1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab209879. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Pin1 Capture Antibody 2 x 50µg 1 x 50µg Mouse Pin1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Pin1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations. -
Sequence similarities
Contains 1 PpiC domain.
Contains 1 WW domain. -
Domain
The WW domain is required for the interaction with STIL and KIF20B. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- DOD
- DODO, Drosophila, homolog of
- FLJ40239
see all -
Database links
- Entrez Gene: 23988 Mouse
- SwissProt: Q9QUR7 Mouse
- Unigene: 7906 Mouse
Images
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Mouse Pin1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Pin1 Matched Antibody Pair Kit (ab218178)To learn more about the advantages of recombinant antibodies see here.