Anti-PLK1 (phospho T210) antibody (ab12157)
Key features and details
- Rabbit polyclonal to PLK1 (phospho T210)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PLK1 (phospho T210) antibody
See all PLK1 primary antibodies -
Description
Rabbit polyclonal to PLK1 (phospho T210) -
Host species
Rabbit -
Specificity
Detects a band at 55kDa in mouse A20 cells that we believe corresponds to Plk1 (predicted molecular weight 68kDa). Also gives positive staining in immunohistochemistry in human colon carcinoma as expected. However, we have been unable to conclusively demonstrate the specificity of the antibody for phospho T210 Plk1. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis, Drosophila melanogaster, Zebrafish
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Immunogen
Synthetic peptide: C-GERKKTPLCGT, corresponding to amino acids 205-214 of Human Plk1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PLK1 (phospho T210) antibody (ab12157)Anti-PLK1 (phospho T210) antibody (ab12157) at 1/500 dilution + Mouse A20 cell lysate
Predicted band size: 68 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa, 40 kDa, 42 kDa. We are unsure as to the identity of these extra bands.
We believe the 55kDa band represents Plk1. However, we cannot be sure that the antibody is detecting phospho T210 Plk1 specifically. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody (ab12157)ab12157 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. Tissue was formalin-fixed and paraffin embedded. Detection with AEC (pink), nuclear counterstaining with Haematoxylin (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody (ab12157)ab12157 (1/200) detecting Plk1 (phospho T210) in formalin-fixed paraffin embedded human breast carcinoma tissue.
