Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4120] to MCM2 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MCM2 antibody [EPR4120] - BSA and Azide free
    See all MCM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR4120] to MCM2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF-7, Ramos, SW480, Molt-4, Jurkat and HeLa cell lysates IHC-P: Human squamous cell cervical carcinoma tissue and Human tonsil tissue IF: HeLa cells

  • General notes

    Ab226044 is the carrier-free version of ab108935. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226044 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 5.80 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4120

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/10000 dilution (unpurified)

    Lane 1 : MCF-7 cell lysate
    Lane 2 : Ramos cell lysate
    Lane 3 : SW480 cell lysate
    Lane 4 : Molt-4 cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 102 kDa



    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Immunocytochemistry - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)

    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling MCM2 with purified ab108935 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)

    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of MCM2 in paraffin-embedded human tonsil tissue with unpurified ab108395, at a 1/250 dilution.
  • Immunocytochemistry - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Immunocytochemistry - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)

    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

    Unpurified ab108935, at a 1/100 dilution, staining MCM2 in HeLa cells.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)

    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin embedded human tonsil with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
  • Immunocytochemistry - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Immunocytochemistry - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)

    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

    Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab108935 at a dilution of 1/600. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
  • Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 102 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)

    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin embedded rat liver with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
  • Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution (purified) + Jurkat cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 102 kDa
    Observed band size: 125 kDa why is the actual band size different from the predicted?



    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Flow Cytometry - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)

    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab108935 at 1/160 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
  • Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Western blot - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/5000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 102 kDa
    Observed band size: 125 kDa why is the actual band size different from the predicted?



    This data was developed using ab108935, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    This data was developed using ab108935, the same antibody clone in a different buffer formulation.Immunohistochemical staining of MCM2 in paraffin-embedded human squamous cell cervical carcinoma tissue with unpurified ab108395, at a 1/250 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    This data was developed using ab108935, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded rat liver with unpurified ab108935 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
  • Immunocytochemistry - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Immunocytochemistry - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    This data was developed using ab108935, the same antibody clone in a different buffer formulation.Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab108935 at a dilution of 1/250. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
  • OI-RD Scanning - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    OI-RD Scanning - Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    This data was developed using ab108935, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)
    Anti-MCM2 antibody [EPR4120] - BSA and Azide free (ab226044)

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