Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1574] to Stathmin 1 (phospho S63)
  • Suitable for: Dot blot, IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Stathmin 1 (phospho S63) antibody [EPR1574]
    See all Stathmin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1574] to Stathmin 1 (phospho S63)

  • Host species

    Rabbit

  • Specificity

    ab76583 only detects Stathmin 1 phosphorylated on Serine 62. The antibody immunogen shares 86% homology with Stathmin-2, therefore it is possible that the antibody will cross-react with Stathmin-2 when phosphorylated at serine 97. This has not been assessed experimentally.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Stathmin 1. The exact sequence is proprietary.

  • Positive control

    • HeLa cell lysates treated with Calyculin A; human brain tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)
    Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)
    All lanes : Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583) at 1/1000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with nocodazole at 100 ng/mL for 18 hours. Whole cell lysates
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with nocodazole at 100 ng/mL for 18 hours. Whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa


    Exposure time: 30 seconds


    Blocking/Diluting buffer and concentration 2% BSA/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    ab76583, at a 1/250 dilution, staining Stathmin 1 in paraffin embedded human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)
    Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)
    All lanes : Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583) at 1/10000 dilution

    Lane 1 : HeLa cell lysate, untreated
    Lane 2 : HeLa cell lysate treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Dot Blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)
    Dot Blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    Dot blot analysis of Stathmin 1 (phospho S63) phospho peptide (Lane 1) and Stathmin 1 non-phospho peptide (Lane 2) labeling Stathmin 1 (phospho S63) with ab76583 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)
    Western blot - Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583) Image courtesy of Qifeng Lin by Abreview.
    Alkaline phosphatase treatment removes S63 phosphorylation. Samples treated with phosphatase were run alongside the normal lysate, and the phosph-S63 signal is not detected after phosphatase treatment, thus suggesting the signal is very specific to the phosphorylated S63.
    Whole cell lysate prepared from a human colon cancer cell line was loaded at 20µg.ab76583 used at a 1/1000 dilution.
    Secondary used was an HRP conjugated goat anti-rabbit polyclonal used at a 1/10000 dilution.

    See Abreview

  • Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)
    Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

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