Anti-Lipocalin-2 / NGAL antibody (ab63929)
Key features and details
- Rabbit polyclonal to Lipocalin-2 / NGAL
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Lipocalin-2 / NGAL antibody
See all Lipocalin-2 / NGAL primary antibodies -
Description
Rabbit polyclonal to Lipocalin-2 / NGAL -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Mouse Lipocalin-2/ NGAL. Residues surrounding amino acid 40.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Lipocalin-2 / NGAL antibody (ab63929) at 1/1000 dilution (12 hours at 4degC) + Rat kidney whole tissue lysate at 10 µg
Secondary
Goat anti-mouse IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 23 kDa
Exposure time: 10 secondsBlocking: 5% milk for 1 hour at 20°C.
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NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling Lipocalin-2 / NGAL using ab63929 (red) in ICC/IF.
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All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 1.5 µg/ml
Lane 1 : Uninduced Jurkat cell lysate
Lane 2 : Induced Jurkat cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 70 kDa (possible non-specific binding)
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All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 2 µg/ml (12 hours at 4deg)
Lanes 1 & 4 : Healthy mouse urine treated with CLB (NP-40 based lysis buffer with protease inhibitors)
Lanes 2-3 : Acute kidney injury mouse urine treated with CLB (NP-40 based lysis buffer with protease inhibitors)
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Donkey anti rabbit HRP at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 1 secondDiluent: TBS - 0.1 % Tween- 5% milk
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ab63929 staining Lipocalin-2 / NGAL in the RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclona l(1/1000) was used as the secondary antibody.
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All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 4 µg/ml
Lane 1 : Recombinant mouse Lipocalin-2/ NGAL protein, 2 ng
Lane 2 : Recombinant mouse Lipocalin-2/ NGAL protein, 10 ng
Lane 3 : Recombinant mouse Lipocalin-2/ NGAL protein, 50 ng
Predicted band size: 23 kDa