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Immunology Adaptive Immunity T Cells Non-CD

Anti-Lipocalin-2 / NGAL antibody (ab63929)

Price and availability

324 988 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Lipocalin-2 / NGAL antibody (ab63929)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Lipocalin-2 / NGAL
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Lipocalin-2 / NGAL antibody
    See all Lipocalin-2 / NGAL primary antibodies
  • Description

    Rabbit polyclonal to Lipocalin-2 / NGAL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Lipocalin-2/ NGAL. Residues surrounding amino acid 40.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Binding proteins
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Tumor biomarkers
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Images

  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929) Verified Customer
    Anti-Lipocalin-2 / NGAL antibody (ab63929) at 1/1000 dilution (12 hours at 4degC) + Rat kidney whole tissue lysate at 10 µg

    Secondary
    Goat anti-mouse IgG-HRP at 1/10000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 23 kDa


    Exposure time: 10 seconds


    Blocking: 5% milk for 1 hour at 20°C.

  • Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)

    NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling Lipocalin-2 / NGAL using ab63929 (red) in ICC/IF.

  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 1.5 µg/ml

    Lane 1 : Uninduced Jurkat cell lysate
    Lane 2 : Induced Jurkat cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa
    Additional bands at: 70 kDa (possible non-specific binding)

  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929) Verified Customer
    All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 2 µg/ml (12 hours at 4deg)

    Lanes 1 & 4 : Healthy mouse urine treated with CLB (NP-40 based lysis buffer with protease inhibitors)
    Lanes 2-3 : Acute kidney injury mouse urine treated with CLB (NP-40 based lysis buffer with protease inhibitors)

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : Donkey anti rabbit HRP at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa


    Exposure time: 1 second


    Diluent: TBS - 0.1 % Tween- 5% milk

  • Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Immunocytochemistry - Anti-Lipocalin-2 / NGAL antibody (ab63929) This image is courtesy of an Abreview submitted by Mahesh Shivananjappa

    ab63929 staining Lipocalin-2 / NGAL in the RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde, permeabilized with Triton 0.1% + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclona l(1/1000) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    Western blot - Anti-Lipocalin-2 / NGAL antibody (ab63929)
    All lanes : Anti-Lipocalin-2 / NGAL antibody (ab63929) at 4 µg/ml

    Lane 1 : Recombinant mouse Lipocalin-2/ NGAL protein, 2 ng
    Lane 2 : Recombinant mouse Lipocalin-2/ NGAL protein, 10 ng
    Lane 3 : Recombinant mouse Lipocalin-2/ NGAL protein, 50 ng

    Predicted band size: 23 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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