Anti-ATOX1 antibody [EPR10352] (ab154179)
![Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-410349-anti-atox1-antibody-epr10352-western-blot-human-knockout-lysate.jpg "Anti-ATOX1 antibody [EPR10352] (ab154179)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10352] to ATOX1
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ATOX1 antibody [EPR10352]
See all ATOX1 primary antibodies -
Description
Rabbit monoclonal [EPR10352] to ATOX1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to amino acids of Human ATOX1 (UniProt ID: O00244).
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Positive control
- WB: HepG2, HeLa and 293T cell lysates. IHC-P: Human prostatic hyperplasia tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10352 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ATOX1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDaLanes 1-4: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab8245 observed at 36 kDa.
ab154179 Anti-ATOX1 antibody [EPR10352] was shown to specifically react with ATOX1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266651 (knockout cell lysate ab257849) was used. Wild-type and ATOX1 knockout samples were subjected to SDS-PAGE. ab154179 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-160112-anti-atox1-antibody-epr10352-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATOX1 antibody [EPR10352] (ab154179)Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling ATOX1 with ab154179 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-414655-anti-atox1-antibody-epr10352-western-blot-hek293-wt-hek293t-atox1-knockout.jpg)
Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : ATOX1 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 7 kDa
Observed band size: 7 kDaLanes 1 - 2: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab154179 was shown to react with ATOX1 in HEK-293 wild-type cells in western blot with loss of signal observed in ATOX1 knockout sample. HEK-293 wild-type and ATOX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab154179 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-160113-anti-atox1-antibody-epr10352-western-blot.jpg)
Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 7 kDa
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![Anti-ATOX1 antibody [EPR10352] (ab154179)](https://www.abcam.com/ps/products/154/ab154179/Images/ab154179-5-benefits-of-recombinant-antibodies.png)
Anti-ATOX1 antibody [EPR10352] (ab154179)
