Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATOX1 antibody [EPR10352] (ab154179)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10352] to ATOX1
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ATOX1 antibody [EPR10352]
    See all ATOX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10352] to ATOX1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to amino acids of Human ATOX1 (UniProt ID: O00244).

  • Positive control

    • WB: HepG2, HeLa and 293T cell lysates. IHC-P: Human prostatic hyperplasia tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)
    Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)
    All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/500 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ATOX1 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 7 kDa
    Observed band size: 7 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab154179 Anti-ATOX1 antibody [EPR10352] was shown to specifically react with ATOX1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266651 (knockout cell lysate ab257849) was used. Wild-type and ATOX1 knockout samples were subjected to SDS-PAGE. ab154179 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATOX1 antibody [EPR10352] (ab154179)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATOX1 antibody [EPR10352] (ab154179)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling ATOX1 with ab154179 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)
    Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)
    All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293 cell lysate
    Lane 2 : ATOX1 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 7 kDa
    Observed band size: 7 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab154179 was shown to react with ATOX1 in HEK-293 wild-type cells in western blot with loss of signal observed in ATOX1 knockout sample. HEK-293 wild-type and ATOX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab154179 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)
    Western blot - Anti-ATOX1 antibody [EPR10352] (ab154179)
    All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution

    Lane 1 : HepG2 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 7 kDa

  • Anti-ATOX1 antibody [EPR10352] (ab154179)
    Anti-ATOX1 antibody [EPR10352] (ab154179)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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