Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free (ab224264)
![Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free (ab224264)](https://www.abcam.com/ps/products/224/ab224264/Images/ab224264-324784-anti-lipocalin-2-ngal-antibody-epr19912-immunohistochemistry.jpg "Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free (ab224264)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19912] to Lipocalin-2 / NGAL - Low endotoxin, Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free
See all Lipocalin-2 / NGAL primary antibodies -
Description
Rabbit monoclonal [EPR19912] to Lipocalin-2 / NGAL - Low endotoxin, Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 whole cell lysates; human ovary cancer, fetal spleen and colon cancer lysates; IHC-P: Human spleen, liver and ovary cancer tissues.
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General notes
ab224264 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19912 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free (ab224264)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Lipocalin-2 / NGAL with ab206427 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neutrophils is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206427).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free (ab224264)](https://www.abcam.com/ps/products/224/ab224264/Images/ab224264-324783-anti-lipocalin-2-ngal-antibody-epr19912-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free (ab224264)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Lipocalin-2 / NGAL with ab206427 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on neutrophils and negative on hepatocytes. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206427).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free (ab224264)](https://www.abcam.com/ps/products/224/ab224264/Images/ab224264-284392-anti-lipocalin-2-ngal-antibody-epr19912-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free (ab224264)This IHC data was generated using the same anti-NGAL antibody clone [EPR19912] in a different buffer formulation (cat# ab20627).
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue labeling Lipocalin-2 / NGAL with ab206427 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human ovary cancer is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free (ab224264)](https://www.abcam.com/ps/products/224/ab224264/Images/ab224264-4-benefits-of-recombinant-antibodies.png)
Anti-Lipocalin-2 / NGAL antibody [EPR19912] - Low endotoxin, Azide free (ab224264)
