Monkey IL-10 ELISA Kit (ab119530)
Key features and details
- Sensitivity: 1.3 U/ml
- Range: 3.9 U/ml - 250 U/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Overview
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Product name
Monkey IL-10 ELISA Kit
See all IL-10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 6 Inter-assay Sample n Mean SD CV% Overall 6 -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
1.3 U/ml -
Range
3.9 U/ml - 250 U/ml -
Recovery
95 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Monkey -
Product overview
Abcam’s IL-10 Monkey ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Monkey IL-10 in cell culture supernatant and serum (baboon, cynomolgus).
This assay employs an antibody specific for Monkey IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Monkey IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Standard is recombinant protein produced in E.coli.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-monkey IL-10 monoclonal antibody 1 x 70µl IL-10 Standard lyophilized (500 U/mL upon reconstitution) 2 vials Microplate coated with monoclonal antibody to IL-10 (12 x 8 wells) 1 unit Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
Research areas
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all
Images
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Typical Standard Curve
Representative Standard Curve using ab119530.