Anti-KAP1 antibody (ab190178)
Key features and details
- Rabbit polyclonal to KAP1
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
Overview
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Product name
Anti-KAP1 antibody
See all KAP1 primary antibodies -
Description
Rabbit polyclonal to KAP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human -
Immunogen
Recombinant fragment corresponding to Human KAP1 aa 400-650.
Database link: Q13263 -
Positive control
- U2OS cells transfected with KAP-1 siRNA, U2OS, mouse embryo fibroblast, rat INS-1 and chicken DT40 cell lysate. U2OS cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-KAP1 antibody (ab190178) at 1/3000 dilution
Lane 1 : Human U2OS cell lysate
Lane 2 : Human U2OS cells transfected with KAP1 siRNA
Lane 3 : Mouse embryo fibroblasts (MEFs) cell lysate
Lane 4 : Rat INS1 cell lysate
Lane 5 : Chicken DT40 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 88 kDa
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Western blot analysis with ab190178 of immunoprecipitated U2OS cells extracts:
Lane 1: Input.
Lane 2: Immunoprecipitation with control serum.
Lane 3: Immunoprecipitation with ab190178 (5 µL).
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Immunofluorescence analysis of U2OS cells labeling KAP1 using ab190178 at a 1/2000 dilution.