Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)
![Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)](https://www.abcam.com/ps/products/241/ab241562/Images/ab241562-355342-anti-lipocalin-2-ngal-hat265b-ihc-p-human-bone-marrow.jpg "Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [HAT265B] to Lipocalin-2 / NGAL
- Suitable for: IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-Lipocalin-2 / NGAL antibody [HAT265B]
See all Lipocalin-2 / NGAL primary antibodies -
Description
Mouse monoclonal [HAT265B] to Lipocalin-2 / NGAL -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
-
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
-
Positive control
- IHC-P: Normal human bone marrow. Human tonsil and spleen tissue. Human gastric carcinoma tissue.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HAT265B -
Isotype
IgG1 -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)
IHC image of Lipocalin 2/NGAL staining in a section of formalin-fixed paraffin-embedded normal human bone marrow* performed on a Leica BOND™ system using the standard Protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab241562, 0.255µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)](https://www.abcam.com/ps/products/241/ab241562/Images/ab241562-356225-AntiLipocalin2--NGAL-antibody-HAT265B-immunohistochemistry-gastric-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling Lipocalin-2 / NGAL with ab241562 at 0.255 µg/ml followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human gastric carcinoma tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)](https://www.abcam.com/ps/products/241/ab241562/Images/ab241562-356224-AntiLipocalin2--NGAL-antibody-HAT265B-immunohistochemistry-spleen-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Lipocalin-2 / NGAL with ab241562 at 0.255 µg/ml followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)](https://www.abcam.com/ps/products/241/ab241562/Images/ab241562-356223-AntiLipocalin2--NGAL-antibody-HAT265B-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Lipocalin-2 / NGAL with ab241562 at 0.255 µg/ml followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)](https://www.abcam.com/ps/products/241/ab241562/Images/ab241562-5-benefits-of-recombinant-antibodies.png)
Anti-Lipocalin-2 / NGAL antibody [HAT265B] (ab241562)
