Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab194690)
Key features and details
- Rabbit polyclonal to Histone H3 (di methyl K27) - ChIP Grade
- Suitable for: Dot blot, IHC-P, WB, ICC/IF, ChIP
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (di methyl K27) antibody - ChIP Grade
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (di methyl K27) - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IHC-P, WB, ICC/IF, ChIPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human Histone H3 (di methyl K27). The exact sequence is proprietary.
Database link: Q16695 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human kidney cancer tissue stained for Histone H3 (di methyl K27) using ab194690 at 1/200 dilution in immunohistochemical analysis.
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Immunofluorescence staining of 293T cells stained for Histone H3 (di methyl K27) with ab194690. Nuclei are labeled with DAPI (Blue).
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All lanes : Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab194690)
Lane 1 : HeLa cell lysate
Lane 2 : Histone H3 protein expressed in E.coli
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Predicted band size: 16 kDaBlocking buffer: 3% nonfat dry milk in TBST. -
Dot-blot analysis of methylation peptides using ab194690 at 1:1000 dilution.
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Chromatin immunoprecipitation analysis of extracts of 293T cell line, using ab194690 and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Paraffin-embedded rat brain tissue stained for Histone H3 (di methyl K27) using ab194690 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse heart tissue stained for Histone H3 (di methyl K27) using ab194690 at 1/100 dilution in immunohistochemical analysis.