Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18914] to Histone H4 (methyl R67)
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Histone H4 (methyl R67) antibody [EPR18914]
See all Histone H4 primary antibodies -
Description
Rabbit monoclonal [EPR18914] to Histone H4 (methyl R67) -
Host species
Rabbit -
Specificity
This antibody detects mono methyl, asymmetric di methyl and symmetric di methyl arginine 67. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human testis lysate; RAW 264.7, F9, NIH/3T3 and HeLa whole cell lysates; mouse spleen lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18914 -
Isotype
IgG -
Research areas
Images
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Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/5000 dilution + Human testis lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/5000 dilution
Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cells) whole cell lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 11 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time:
Lane 1 & 2: 4 seconds; Lane 3: 15 seconds.
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