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Epigenetics and Nuclear Signaling Histones H3 Methylated

Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18914] to Histone H4 (methyl R67)
  • Suitable for: WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Histone H4 (methyl R67) antibody [EPR18914]
    See all Histone H4 primary antibodies
  • Description

    Rabbit monoclonal [EPR18914] to Histone H4 (methyl R67)
  • Host species

    Rabbit
  • Specificity

    This antibody detects mono methyl, asymmetric di methyl and symmetric di methyl arginine 67.
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis lysate; RAW 264.7, F9, NIH/3T3 and HeLa whole cell lysates; mouse spleen lysate.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18914
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Methylated

Images

  • Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)
    Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)
    Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/5000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)
    Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)
    All lanes : Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/5000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)
    Western blot - Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)
    All lanes : Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cells) whole cell lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa
    Observed band size: 11 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time:

    Lane 1 & 2: 4 seconds; Lane 3: 15 seconds.

     

  • Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)
    Anti-Histone H4 (methyl R67) antibody [EPR18914] (ab177827)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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